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Acta Agronomica Sinica ›› 2026, Vol. 52 ›› Issue (6): 1830-1846.doi: 10.3724/SP.J.1006.2026.51088

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation

Hu Chuan1(), Zhao Kai-Nan2, Huang Xiu-Li1, Wu Jin-Zhi1, Ren Kai-Ming1, Wang He-Zheng1, Fu Guo-Zhan1, Huang Ming1,*(), Li You-Jun1,*()   

  1. 1 College of Agriculture, Henan University of Science and Technology, Luoyang 471023, Henan, China
    2 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China
  • Received:2025-10-16 Accepted:2026-02-27 Online:2026-06-12 Published:2026-03-09
  • Contact: * Huang Ming, E-mail: huangming_2003@126.com;Li You-Jun, E-mail: lyj@haust.edu.cn
  • Supported by:
    National Key Research and Development Program of China(2021YFD1900700);National Key Research and Development Program of China(2022YFD2300800);Science and Technology Research Project of Henan(252102111080);Science and Technology Research Project of Henan(232102111009)

Abstract:

As China’s High-Standard Farmland Construction Program advances, many dryland wheat fields can now receive a one-off irrigation during the growing season (hereafter, one-off irrigation). To evaluate the effects of tillage practices and nitrogen (N) rates on wheat yield and quality under one-off irrigation, we conducted a two-factor split-plot experiment from 2020 to 2022 at three sites in Luoyang, Henan province (Xiaolangdi town, Mengjin county; Yaling town, Yichuan county; and Xiaojie town, Luoning county). Tillage practice was the main-plot factor—rotary tillage (RT), subsoiling (SS), and plough tillage (PT)—and N rate was the subplot factor at 0 (N0), 120 (N120), 180 (N180), and 240 (N240) kg hm-2. We measured grain yield, protein content and protein fractions, grain Zn content, and key processing-quality traits. Tillage practice and N rate significantly affected wheat yield and quality; their interaction significantly influenced grain yield, protein yield, and the contents of all protein components except albumin. Compared with PT and RT, SS increased grain yield by 6.9% and 12.6%, respectively, and increased protein yield by 7.7% and 14.5%, while generally improving protein content, most protein fractions, processing quality, and grain Zn content (with a few site-year exceptions). Relative to PT, SS increased albumin, globulin, gliadin, and glutenin contents as well as dough development time, stability time, wet gluten content, sedimentation value, extensibility, maximum resistance, and grain Zn content by 21.9%, 19.0%, 12.5%, 8.0%, 18.6%, 28.4%, 8.2%, 26.4%, 10.1%, 14.0%, and 12.6%, respectively; compared with RT, the corresponding increases were 22.0%, 19.6%, 19.7%, 15.0%, 19.5%, 32.8%, 9.1%, 27.8%, 10.3%, 22.6%, and 23.2%, respectively. Across all tillage practices, increasing N rate led to an initial increase followed by a plateau in yield, protein yield, protein fractions, processing-quality traits, and grain Zn content. In most cases, N180 and N240 did not differ significantly, and both outperformed N120. Except for grain yield at the Xiaolangdi site and gliadin content in 2021-2022, SSN180 achieved similar yield, quality, economic returns, and input-output ratio to SSN240, while outperforming the other treatments in most comparisons. Overall, subsoiling combined with 180 kg hm-2 N is recommended for dryland regions where one-off irrigation is available, as it can simultaneously improve wheat yield, quality, and economic benefits.

Key words: tillage methods, nitrogen rates, dryland, wheat, yield, quality

Table 1

Soil physical and chemical properties in the 0-20 cm soil layer at the start of the experiment"

试验地点
Experimental site
有机质含量
Organic matter content
(g kg-1)
全氮含量
Total N
content
(mg kg-1)
硝态氮含量
Nitrate N content
(mg kg-1)
有效磷含量
Available P content
(mg kg-1)
速效钾含量
Available K
content
(mg kg-1)
pH 土壤容重
Soil bulk density
(g cm-3)
田间持水量
Field
capacity
(%)
小浪底Xiaolangdi 14.7 1.11 19.36 9.0 139.6 7.57 1.35 27.4
鸦岭Yaling 12.4 1.08 8.46 12.7 177.1 7.97 1.40 26.0
小界Xiaojie 13.2 0.83 6.63 5.9 91.5 7.98 1.26 25.3

Fig. 1

Monthly precipitation and mean temperature at the experimental sites from June 2020 to May 2022"

Table 2

The variance analysis of wheat yield and protein yield at different experimental sites"

变异来源
Source of
variance
产量Yield 蛋白质产量Protein yield
小浪底
Xiaolangdi
鸦岭
Yaling
小界
Xiaojie
3点均值
3-sites average
小浪底
Xiaolangdi
鸦岭
Yaling
小界
Xiaojie
3点均值
3-sites average
Y 232.8** 276.4** 3747.9** 0 130.1** 120.1** 5591.0** 1.9
T 40.8** 512.0** 172.9** 55.0** 174.0** 515.8** 25.0** 160.8**
N 750.0** 292.7** 1380.8** 277.3** 640.7** 240.6** 813.8** 3821.8**
Y×T 1.4 16.7** 20.2** 1.6 0.5 8.8** 2.3 4.1
Y×N 33.9** 3.4* 21.1** 2.3 15.1** 0.2 13.5** 36.3**
T×N 2.5* 2.9* 7.9** 1.0 17.7** 1.8 4.2** 17.4**
Y×T×N 1.7* 1.8 11.7** 0.4 0.6 0.5 2.2 0.9

Fig. 2

Effects of different treatments on grain yield and protein yield of dryland wheat under one-off irrigation RT, SS, and PT denote rotary tillage, subsoiling + rotary tillage, and plough tillage + rotary tillage, respectively. N0, N120, N180, and N240 denote N rates of 0, 120, 180, and 240 kg hm-2, respectively. Error bars indicate standard deviation. Different lowercase letters indicate significant differences among treatments at the same site in the same year (P < 0.05)."

Table S1

ANOVA of the effects of different treatments on crude protein and protein fractions in dryland wheat grain under one-off irrigation"

变异来源
Source of variance
粗蛋白含量Crude protein (%) 清蛋白含量Albumin (%) 球蛋白含量Globulin (%)
小浪底
Xiaolangdi
鸦岭
Yaling
小界
Xiaojie
小浪底
Xiaolangdi
鸦岭
Yaling
小界
Xiaojie
小浪底
Xiaolangdi
鸦岭
Yaling
小界
Xiaojie
Y 326.26** 44.48* 0.28 31.97* 393.92* 2.29 0.14 24.29* 23.54*
T 22.55** 1.61 0.13 18.40** 39.92** 1.04 22.64** 9.22** 9.89*
N 214.23** 60.85** 130.42** 20.33** 6.09** 24.68** 48.97** 7.11** 23.74**
Y×T 3.51 22.35** 0.02 0.48 14.42** 0.27 4.64* 0.77 2.09**
Y×N 4.22* 2.37 15.25** 0.50 0.22 0.20 1.22 0.86 2.11
T×N 13.67** 1.31 0.62 1.66 1.27 1.08 4.61** 1.78 3.16*
Y×T×N 1.54 1.19 0.70 1.74 0.40 0.33 1.99 0.78 0.30
变异来源
Source of variance
醇溶蛋白含量Gliadin (%) 谷蛋白含量Glutenin (%)
小浪底
Xiaolangdi
鸦岭
Yaling
小界
Xiaojie
小浪底
Xiaolangdi
鸦岭
Yaling
小界
Xiaojie
Y 62.92* 31.36* 10.81 0.38 25.07* 50.26*
T 14.61** 103.06** 90.09** 27.64** 22.85** 11.71**
N 15.80** 43.66** 49.43** 50.79** 76.31** 14.24**
Y×T 0.06 0.60 5.56 4.06 0.61 0.23
Y×N 1.01 2.46 0.93 0.45 1.98 0.90
T×N 2.69* 3.65** 3.52** 5.43** 4.96** 0.34
Y×T×N 0.45 1.14 0.17 1.04 1.84 0.56

Fig. 3

Effects of different treatments on crude protein and protein fractions in dryland wheat grain under one-off irrigation Treatments are the same as those given in Fig. 2. In the boxplots, the upper and lower boundaries indicate the third and first quartiles, respectively; the line within the box indicates the median; and the box height represents the interquartile range. Different lowercase letters indicate significant differences among treatments (P < 0.05)."

Table S2

ANOVA of the effects of different treatments on grain processing-quality traits of dryland wheat under one-off irrigation"

变异来源
Source of
variance
形成时间Development time (min) 稳定时间Stability time (min) 湿面筋含量Wet gluten content (%)
小浪底Xiaolangdi 鸦岭Yaling 小界Xiaojie 小浪底Xiaolangdi 鸦岭Yaling 小界Xiaojie 小浪底Xiaolangdi 鸦岭Yaling 小界Xiaojie
Y 20.39* 1280.8** 94.31* 459.85** 3002.07** 26.75* 130.6** 1178.35** 34.21*
T 22.58** 118.53** 2.52 122.25** 14.76** 93.89** 22.82** 25.42** 1.03
N 27.90** 64.80** 289.86** 92.97** 22.59** 51.01** 169.95** 43.11** 98.96**
Y×T 0.20 18.06** 1.21 14.87** 0.00 3.04 1.53 4.19 0.15
Y×N 1.00 1.77 1.03 3.34* 4.21* 6.87** 3.93* 0.23 0.25
T×N 1.51 2.14 4.39** 6.24** 0.65 2.28 6.00** 1.54 1.85
Y×T×N 0.25 2.31 3.59** 1.26 0.32 0.68 0.91 1.59 1.12
Y 135.27** 646.95** 75.06* 1660.10** 1578.18** 102.94** 692.61** 15.37 168.59**
T 30.59** 24.49** 9.72** 440.44** 47.63** 6.80* 12.98** 56.51** 23.03**
N 183.39** 49.76** 76.33** 104.22** 27.09** 77.62** 19.60** 15.59** 13.70**
Y×T 0.01 6.20* 0.32 4.94* 4.26 1.23 0.11 0.81 3.00
Y×N 0.14 8.90** 2.06 5.15** 0.74 10.80** 0.18 0.34 2.05
T×N 2.08 1.32 1.42 0.63 1.33 1.99 0.15 0.31 2.48*
Y×T×N 5.04** 1.47 0.44 3.57** 3.61** 1.53 0.06 0.25 1.25

Fig. 4

Effects of different treatments on grain processing-quality traits of dryland wheat under one-off irrigation Treatments are the same as those given in Fig. 2. In the boxplots, the upper and lower boundaries indicate the third and first quartiles, respectively; the line within the box indicates the median; and the box height represents the interquartile range. Different lowercase letters indicate significant differences among treatments (P < 0.05)."

Table 3

The variance analysis of zinc content in wheat grain at different experimental sites"

变异来源
Source of variance
小浪底
Xiaolangdi
鸦岭
Yaling
小界
Xiaojie
3点均值
3-site
average
Y 175.74** 282.75** 18.47 11.39**
T 15.17** 7.24* 10.23** 25.76**
N 50.75** 16.05** 14.21** 60.93**
Y×T 4.41 1.59 0.34 0.78
Y×N 16.81** 0.79 0.19 3.78*
T×N 2.00 0.91 0.66 1.86
Y×T×N 0.88 0.26 0.31 0.31

Fig. 5

Effects of different treatments on grain Zn content of dryland wheat under one-off irrigation Treatments are the same as those given in Fig. 2. Different lowercase letters indicate significant differences among treatments at the same site in the same year (P < 0.05). Error bars indicate standard deviation."

Fig. 6

Correlations among grain yield, protein traits, processing-quality indicators, and Zn content in wheat under one-off irrigation Yield, AL, Glo, Gli, Glu, TO, DT, ST, WG, SV, EX, MR, and Zn denote grain yield, albumin content, globulin content, gliadin content, glutenin content, crude protein content, dough development time, dough stability time, wet gluten content, sedimentation value, extensibility, maximum resistance, and Zn content, respectively. * and ** indicate significant correlations at P < 0.05 and P < 0.01, respectively."

Table 4

Effects of different treatments on the economic returns of dryland wheat under one-off irrigation"

年度
Year
处理
Treatment
小浪底Xiaolangdi 鸦岭Yaling 小界Xiaojie
经济效益
Economic
benefits
(Yuan hm-2)
产投比
Input-output ratio
经济效益
Economic
benefits
(Yuan hm-2)
产投比
Input-output ratio
经济效益
Economic
benefits
(Yuan hm-2)
产投比
Input-output ratio
2020-2021 RTN0 11,971 f 3.27 g 10,567 e 3.01 g 5491 g 2.04 f
RTN120 14,315 d 3.49 cd 11,932 d 3.08 efg 10,027 f 2.75 d
RTN180 15,334 bc 3.57 bc 14,034 bc 3.35 c 12,275 d 3.06 c
RTN240 15,692 b 3.53 bc 13,562 c 3.18 de 10,882 e 2.75 d
SSN0 13,158 e 3.50 cd 12,500 d 3.37 c 6354 g 2.21 e
SSN120 15,023 c 3.62 b 15,853 a 3.76 a 12,042 d 3.10 c
SSN180 16,532 a 3.77 a 16,324 a 3.73 ab 16,225 ab 3.72 a
SSN240 16,858 a 3.72 a 16,299 a 3.63 b 16,524 a 3.66 a
PTN0 12,089 f 3.13 h 10,457 e 2.84 h 5578 g 1.98 f
PTN120 14,216 d 3.31 fg 12,599 d 3.05 fg 11,562 de 2.88 d
PTN180 15,449 bc 3.42 de 14,113 bc 3.21 d 15,357 c 3.40 b
PTN240 15,688 c 3.37 ef 14,336 b 3.17 def 15,519 bc 3.34 b
2021-2022 RTN0 8840 c 2.68 c 9476 e 2.80 de 8476 g 2.61 f
RTN120 13,465 b 3.35 a 11,332 c 2.97 bc 15,179 e 3.65 bcd
RTN180 14,141 b 3.37 a 11,722 c 2.96 bc 16,930 abc 3.83 a
RTN240 13,470 b 3.17 b 11,671 c 2.88 cd 16,184 cd 3.61 bcd
SSN0 9294 c 2.61 c 12,073 c 3.10 b 10,066 f 2.75 e
SSN120 14,019 b 3.25 ab 14,059 b 3.26 a 16,917 abc 3.72 abc
SSN180 15,082 a 3.33 a 14,858 a 3.30 a 17,641 a 3.73 ab
SSN240 15,686 a 3.34 a 14,987 a 3.24 a 17,680 a 3.64 bcd
PTN0 9110 c 2.60 c 8975 e 2.58 f 9729 f 2.71 ef
PTN120 13,387 b 3.18 b 10,437 d 2.70 ef 15,768 de 3.56 d
PTN180 13,955 b 3.19 b 12,106 c 2.90 cd 16,631 bc 3.60 bcd
PTN240 14,043 b 3.12 b 12,223 c 2.85 cd 17,104 ab 3.58 cd
方差分析
ANOVA
年度Year (Y) 281.3** 406.5** 344.4** 437.6** 3403.5** 2193.4**
耕作方式Tillage (T) 32.5** 51.6** 485.3** 476.8** 133.7** 72.7**
氮肥用量Nitrogen (N) 432.9** 153.1** 220.9** 51.5** 1527.7** 1054.8**
Y×T 0.1 14.5** 5.9* 9.3** 25.4** 55.2**
Y×N 27.5** 32.7** 4.1* 2.5 28.2** 36.0**
T×N 2.9* 2.3 2.6* 2.8* 7.0** 4.9**
Y×T×N 2.0 2.0 1.7 2.0 10.4** 10.2**
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