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Acta Agronomica Sinica ›› 2026, Vol. 52 ›› Issue (6): 1802-1816.doi: 10.3724/SP.J.1006.2026.53066

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of tillage methods and nitrogen application rate on maize yield and soil aggregates in northeastern China under straw returning

Ma Sheng-Qian1,2(), Wang Zhi-Ping1,2, Chen Hao-Tian2, Dou Shu-Xian2,3, Zhang Yan2, Deng Ai-Xing2, Zhang Wei-Jian2, Yuan Xiang-Yang1,*(), Song Zhen-Wei2,*()   

  1. 1 College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China
    2 Institute of Crop Science, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
    3 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
  • Received:2025-08-25 Accepted:2026-03-16 Online:2026-06-12 Published:2026-03-20
  • Contact: * Yuan Xiang-Yang, E-mail: yuanxiangyang200@163.com;Song Zhen-Wei, E-mail: songzhenwei@caas.cn
  • Supported by:
    National Key Research and Development Program(2022YFD1500702);Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202202)

Abstract:

Northeastern China is a major maize-producing region, but decades of unsustainable tillage practices and excessive nitrogen fertilization have degraded black soils and threatened the sustainable production of maize. Conservation tillage practices based on straw return and reduced or no tillage are key approaches to improving soil quality and maintaining crop productivity, and optimizing nitrogen inputs is essential for enhancing resource-use efficiency. To determine suitable tillage practices and optimal nitrogen rates under straw incorporation, and to promote coordinated improvements in maize yield and soil quality in northeastern China, a fixed-site field experiment was conducted from 2022 to 2024 at Yanjiagang farm, Harbin, Heilongjiang province. The experiment used a split-plot design with tillage as the main-plot factor—conventional tillage (CT), rotary tillage (RT), strip tillage (ST), and no tillage (NT)—and nitrogen rate as the subplot factor: 0 (N0), 135 (N1), 180 (N2), and 225 kg hm-2 (N3) as pure N. Across the three years, mean grain yield ranked ST > CT > RT > NT, although differences among tillage treatments were not significant. Relative to NT, ST increased yield by 14.5% in 2023 and 6.6% in 2024, with no significant differences between ST and CT or RT. Nitrogen rate significantly increased yield (P < 0.001); yield under N2 and N3 were 183.1%-217.2% higher than under N0. No significant tillage×nitrogen interaction was detected for yield. Mean soil bulk density in the 0-20 cm layer followed the order NT > ST > RT > CT, with significant differences among tillage treatments; NT was 20.2%-31.4% higher than CT. Both tillage and nitrogen rate significantly affected aggregate-size distribution, mean weight diameter (MWD), and geometric mean diameter (GMD) (P < 0.05). NT increased the proportion of 0.250-2.000 mm aggregates in the 0-20 cm layer by 23.7%-56.7%, and MWD and GMD ranked NT > RT > ST > CT. The N2 and N3 treatments increased the proportion of 0.250-2.000 mm aggregates by 9.2%-29.7% and decreased the proportion of < 0.053 mm aggregates by 30.3%-51.5%. Correlation analysis showed significant positive associations between tillage practice and nitrogen rate under straw return and maize yield (P < 0.01), and the ST treatment achieved the maximum yield at an estimated N rate of 200.2 kg hm-2. Overall, under straw incorporation, strip tillage combined with 180-225 kg hm-2 N can achieve high maize yield while improving soil aggregation, and is therefore recommended for the cold regions of northeastern China.

Key words: straw returning, conservation tillage, nitrogen application rate, soil aggregates, grain yield

Fig. 1

Mean air temperature (℃) and precipitation (mm) in the experimental site from May to October during 2022-2024"

Table 1

Operational details of tillage methods"

耕作方式
Tillage method
操作细节
Operation details
翻耕
Conventional tillage
(CT)
当年秋季进行翻耕作业, 将秸秆翻至土壤30 cm以下, 并进行耙地打破较大的块状土壤并平整土地, 第2年播种前进行起垄作业, 采用常规播种机进行播种与施肥一体化作业
In the autumn, plowing operations were carried out to turn the straw into the soil below 30 cm, followed breaking up large clods of soil and harrowing to level the land surface. Before sowing in the following year, ridging operations were conducted, and conventional seeders were used for sowing and applying fertilizer
旋耕
Rotary tillage
(RT)
当年秋季进行旋耕作业, 将秸秆旋耕至0-20 cm土壤层内, 第2年播种前进行起垄作业, 并采用常规播种机进行播种与施肥一体化作业
In the autumn, rotary tillage was performed to incorporate the straw into the 0-20 cm soil layer. Before sowing in the following year, ridging operations were carried out, and conventional seeders were used for sowing and applying fertilizer
条带耕作
Strip tillage
(ST)
当年秋季采用条耕机在玉米行间进行旋耕作业, 将秸秆旋耕至0-15 cm土壤层内, 旋耕带宽20 cm左右, 其余土壤保持免耕秸秆覆盖, 第2年春季采用免耕播种机进行播种与施肥一体化作业
In the autumn, a strip-till machine was used to perform rotary tillage between the corn rows, incorporating the straw into the 0-15 cm soil layer. The tilled strip width was approximately 20 cm, while the remaining soil was maintained under no-till with straw mulch. In the spring of the following year, a no-till planter was used for sowing and applying fertilizer
免耕
No tillage
(NT)
保持秸秆覆盖不进行任何耕作处理, 第2年春季采用免耕播种机进行播种与施肥一体化作业
Maintain straw mulch without any tillage, and in the spring of the following year, use a no-till planter for direct seeding and applying fertilizer

Table 2

Effects of tillage methods and nitrogen application rate on plant traits of maize from 2022 to 2024"

耕作方式
Tillage method
氮肥施用量
N application rate
株高
Plant height (cm)
穗位高
Ear height (cm)
茎粗
Stem diameter (cm)
V3 V6 R1 R6 R6
CT N0 22.4±0.7 abc 130.2±1.8 bc 278.4±1.4 c 112.6±0.9 cdef 2.4±0.1 de
N1 22.7±0.6 abc 137.3±3.8 a 297.9±4.8 ab 120.4±0.2 ab 2.6±0.0 bc
N2 24.6±0.2 abc 142.0±1.2 a 309.4±4.3 a 127.5±2.2 a 2.6±0.0 bc
N3 26.0±0.6 ab 143.0±0.9 a 306.8±1.9 ab 120.1±3.4 b 2.8±0.1 a
RT N0 21.1±0.1 bc 107.5±1.1 d 259.8±2.7 d 117.0±2.2 bcde 2.2±0.1 f
N1 21.8±0.6 abc 137.5±4.2 a 301.3±6.1 ab 116.5±3.2 bcde 2.5±0.1 cd
N2 21.3±0.7 bc 138.5±2.6 a 308.6±1.0 a 117.7±2.6 bcde 2.7±0.0 b
N3 24.1±1.1 abc 141.8±1.6 a 306.4±6.2 ab 110.7±1.6 ef 2.6±0.1 bc
ST N0 27.0±7.1 a 109.3±3.0 d 252.2±4.7 d 111.5±4.3 def 2.3±0.0 e
N1 24.6±0.5 abc 126.2±2.4 c 295.8±4.3 b 118.2±1.9 bcd 2.5±0.0 cd
N2 22.9±0.3 abc 128.2±4.2 c 301.0±3.3 ab 119.5±2.8 bc 2.7±0.1 b
N3 22.9±0.4 abc 125.7±0.8 c 299.7±3.1 ab 117.7±3.8 bcde 2.7±0.0 b
NT N0 20.2±0.2 c 111.5±2.5 d 257.7±2.7 d 109.3±2.5 f 2.2±0.0 ef
N1 22.5±0.5 abc 136.5±1.0 ab 298.4±1.6 ab 123.2±0.7 ab 2.6±0.0 bc
N2 22.0±0.6 abc 136.2±0.9 ab 307.8±1.1 a 119.8±1.3 bc 2.6±0.0 bc
N3 22.4±0.1 abc 140.2±2.3 a 301.3±7.8 ab 117.5±2.0 bcde 2.7±0.0 b
ANOVA
Tillage method (T) ns *** ns ns ns
N application rate (N) ns *** *** *** ***
T×N ns ** ns * ns

Table 3

Effects of tillage methods and nitrogen application rate on leaf area index of maize from 2022 to 2024"

耕作方式
Tillage method
氮肥施用量
N application rate
V3 V6 R1 R3 R6
CT N0 0.05±0.00 bc 2.12±0.07 f 5.05±0.07 bcd 4.42±0.10 e 2.68±0.03 ef
N1 0.05±0.00 bc 2.42±0.15 e 5.61±0.06 a 5.29±0.06 bc 3.25±0.03 bcd
N2 0.05±0.00 ab 2.70±0.01 d 5.40±0.17 ab 5.42±0.10 abc 2.74±0.08 def
N3 0.06±0.00 a 3.08±0.02 ab 5.31±0.06 ab 5.45±0.06 ab 2.97±0.12 cdef
RT N0 0.03±0.00 f 1.93±0.07 f 4.61±0.07 e 4.61±0.09 de 2.48±0.06 f
N1 0.04±0.00 cde 3.18±0.07 a 5.39±0.25 ab 5.42±0.07 abc 2.69±0.14 ef
N2 0.04±0.00 cde 2.98±0.07 abc 5.26±0.16 abc 5.60±0.11 a 3.04±0.05 cde
N3 0.04±0.00 bcd 3.10±0.05 ab 5.56±0.12 a 5.45±0.05 ab 2.87±0.16 def
ST N0 0.03±0.00 f 2.42±0.07 e 4.92±0.13 cde 4.60±0.01 de 2.49±0.20 f
N1 0.04±0.00 def 2.79±0.08 cd 5.62±0.13 a 5.27±0.02 bc 3.19±0.21 bcde
N2 0.04±0.00 cde 2.96±0.24 abcd 5.35±0.19 ab 5.30±0.07 bc 3.44±0.17 abc
N3 0.04±0.00 cde 2.89±0.03 bcd 5.44±0.13 a 5.23±0.03 c 3.69±0.27 ab
NT N0 0.03±0.00 f 2.38±0.07 e 4.80±0.04 de 4.64±0.09 d 2.43±0.15 f
N1 0.03±0.00 ef 3.06±0.04 ab 5.37±0.04 ab 5.30±0.06 bc 3.60±0.22 ab
N2 0.04±0.00 def 3.20±0.04 a 5.56±0.17 a 5.24±0.09 c 3.86±0.20 a
N3 0.03±0.00 f 3.16±0.06 a 5.55±0.05 a 5.33±0.06 bc 3.48±0.28 abc
ANOVA
Tillage method (T) *** ** ns ns ns
N application rate (N) *** *** *** *** ***
T×N ns ** ns ns **

Table 4

Effects of tillage methods and nitrogen application rate on dry matter accumulation of maize from 2022 to 2024"

耕作方式
Tillage method
氮肥施用量
N application rate
V3 V6 R1 R3 R6
CT N0 17.7±1.6 e 1681.2±94.7 fg 6187.5±224.6 e 9425.3±309.4 d 14,913.4±145.6 e
N1 21.7±1.1 cd 2149.4±214.3 cde 10,142.3±124.7 ab 15,097.4±456.9 c 24,443.4±192.9 bcd
N2 28.2±1.0 b 2174.5±69.4 cde 9610.1±510.3 bcd 15,519.6±537.3 bc 24,579.7±651.5 bcd
N3 32.6±1.9 a 2840.4±71.9 a 10,531.4±395.1 a 17,322.4±253.4 a 25,549.8±752.2 bc
RT N0 13.0±0.8 f 992.1±6.6 i 5226.1±114.4 f 9513.9±459.3 d 12,006.1±271.8 f
N1 21.3±1.0 cd 2223.0±93.1 bcde 9767.5±431.8 abc 15,663.1±441.5 bc 23,574.3±591.5 cd
N2 20.1±1.3 de 2098.8±98.0 de 9846.6±73.9 abc 16,314.7±576.1 abc 24,087.2±703.4 cd
N3 24.1±0.9 c 2528.3±35.3 ab 10,277.8±347.2 ab 16,254.9±541.7 abc 24,891.7±903.2 bcd
ST N0 11.4±0.5 f 1431.9±87.3 gh 5386.6±288.1 ef 9403.2±248.1 d 14,757.7±780.0 e
N1 17.1±0.5 e 1946.4±70.7 ef 8950.9±501.4 cd 15,096.1±434.4 c 22,692.4±660.5 d
N2 18.4±1.6 de 2049.8±239.1 de 10,614.7±247.0 a 16,816.6±260.4 ab 28,052.2±971.0 a
N3 17.3±1.4 e 1896.6±159.4 ef 9716.1±438.6 abcd 15,629.8±782.2 bc 26,897.5±987.1 ab
NT N0 11.9±1.1 f 1135.2±80.1 hi 4971.0±159.4 f 9424.4±266.4 d 12,737.0±265.6 ef
N1 16.7±1.6 e 2349.1±87.5 bcd 8818.5±231.6 d 15,550.5±134.7 bc 22,404.0±342.1 d
N2 17.7±1.0 e 2482.4±204.6 bc 9592.5±139.6 bcd 15,637.2±715.1 bc 24,450.5±869.6 bcd
N3 18.6±0.4 de 2380.5±52.2 bcd 10,014.5±284.4 ab 16,322.6±753.4 abc 25,414.5±196.6 bc
ANOVA
Tillage method (T) *** * ns ns *
N application rate (N) *** *** *** *** ***
T×N ** ** * ns ns

Table 5

Effects of tillage methods and nitrogen application rate on yield and yield components of maize from 2022 to 2024"

耕作方式
Tillage method
氮肥施用量
N application rate
穗数
Ear number
(×104 hm-2)
穗粒数
Grain number
(Number per ear)
百粒重
100-kernal weight
(g)
产量
Grain yield
(kg hm-2)
CT N0 5.0±0.1 d 466.2±20.2 bc 36.2±1.4 e 6219.6±103.0 h
N1 5.9±0.2 abc 682.1±5.2 a 42.3±0.5 cd 12,170.4±238.7 f
N2 5.7±0.1 bc 710.8±22.1 a 43.0±0.3 bcd 13,357.4±230.7 cd
N3 6.1±0.1 ab 700.0±4.5 a 45.1±0.5 ab 14,791.9±87.3 a
RT N0 5.1±0.2 d 461.7±22.4 c 38.5±0.5 e 5772.7±271.3 h
N1 6.0±0.2 abc 662.9±13.8 a 43.6±0.7 bcd 12,414.8±145.3 ef
N2 6.0±0.0 ab 686.5±15.0 a 42.2±1.6 d 13,137.5±90.5 de
N3 6.0±0.1 abc 693.0±6.4 a 44.6±0.1 abc 14,046.4±193.4 abc
ST N0 5.6±0.1 c 525.0±63.5 b 38.0±0.2 e 7244.7±272.7 g
N1 5.9±0.1 abc 686.1±9.2 a 44.7±0.7 abc 13,343.1±285.6 cd
N2 6.2±0.1 a 676.9±7.1 a 45.1±0.5 ab 14,431.8±179.1 ab
N3 5.9±0.1 abc 701.4±10.2 a 44.1±1.6 abcd 14,044.9±266.7 abc
NT N0 4.9±0.1 d 455.6±29.0 c 37.7±0.6 e 5854.6±366.6 h
N1 5.8±0.1 abc 676.1±20.2 a 42.6±0.5 cd 12,548.7±224.6 ef
N2 5.9±0.2 abc 695.9±5.0 a 43.6±1.0 bcd 13,773.2±589.5 bcd
N3 6.0±0.1 abc 674.8±13.0 a 46.0±0.5 a 13,904.0±242.8 bc
ANOVA
Tillage method (T) ns ns * ns
N application rate (N) *** *** *** ***
T×N * ns * ns

Fig. 2

Correlation analysis between nitrogen application rate and maize yield under different tillage methods with straw returning in northeastern China Treatments are the same as those given in Table 2."

Fig. 3

Effects of tillage methods and nitrogen application rate on soil bulk density from 2022 to 2024 Treatments are the same as those given in Table 2. Different letters indicate significant differences among treatments (LSD test, P < 0.05)."

Table 6

ANOVA of the effects of tillage methods and nitrogen application rate on soil aggregates in 2024"

ANOVA 土层深度
Soil depth (cm)
> 2.000 mm
(%)
0.250-2.000 mm
(%)
0.053-0.250 mm
(%)
< 0.053 mm
(%)
MWD GMD
Tillage method (T) 0-20 * * ns ns * *
N application rate (N) ns * ** *** * *
T×N ns ns ns ns ns ns
Tillage method (T) 20-40 ns ns ns ns ns ns
N application rate (N) ns ns * ns ns ns
T×N ns ns ns ns ns ns

Fig. 4

Effects of tillage methods and nitrogen application rate on soil aggregates in 2024 Treatments are the same as those given in Table 2. Different letters indicate significant differences among treatments (LSD test, P < 0.05)."

Fig. 5

Structural equation model showing the effects of tillage methods and nitrogen application rate on maize yield and soil physical properties in northeastern China under straw returning DMA: dry matter accumulation; LAI: leaf area index; BD: soil bulk density; SA: soil aggregates; GY: grain yield. Solid lines indicate positive relationships, and line thickness is proportional to the absolute value of the path coefficient. Dashed lines indicate negative relationships. *: P < 0.05; **: P < 0.01; ***; P < 0.001; ns: no significant difference."

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