%A NAN Hai-Yang,LI Ying-Hui,CHANG Ru-Zhen,QIU Li-Juan %T Development and Identification of InDel Marker Based on rhg1 Gene for Resistance to Soybean Cyst Nematode(Heterodera glycines Ichinohe) %0 Journal Article %D 2009 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2009.01236 %P 1236-1243 %V 35 %N 7 %U {https://zwxb.chinacrops.org/CN/abstract/article_4242.shtml} %8 2009-07-12 %X
Soybean cyst nematode (Heterodera glycaines, SCN) is one of the most important pests of soybean in the world. New molecular markers developed from resistance genes can provide marker resources for MAS (marker-assisted selection) breeding. Among four InDel loci detectedby comparing thesequences of candidate rhg1 conferringSCN resistance, three multiple-bases InDel loci were used to develop markers. A total of 11 alleles were detected in 33 cultivated soybean germplasm using three InDel markers developed. There were 5 alleles at rhg1-I1 locus, 2 and 4 at rhg1-I2 and rhg1-I4 respectively. Allelic variations at three InDel markers were assayed in the genotypes and discovered that rhg1-I4 marker was related to SCN resistance, which could distinguish 88.2% of resistant germplasm and 100% of the susceptible germplasm. Alleles of 288 and 294 bp presented in the SCN-resistant germplasm, and alleles 269 and 272 bp presented in the SCN-susceptible germplasm. In addition, rhg1-I4 could be used in MAS for the progenies of cross between typical southern US cultivar Lee crossed and the important resistant source PI88788. Combing with Satt309 which displayed the identical allele on these genotypes, rhg1-I4 would be higher effective in identifying germplasm with rhg1 conferring resistance in the MAS breeding.