%A MIN Dong-Hong,ZHAO Yue,CHEN Yang,XU Zhao-Shi,HUO Dong-Ying,HU Di,CHEN Ming,LI Lian-Cheng,MA You-Zhi %T Isolation and Molecular Characterization of Stress-Related TaLEAL3 Gene in Wheat %0 Journal Article %D 2012 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2012.01847 %P 1847-1855 %V 38 %N 10 %U {https://zwxb.chinacrops.org/CN/abstract/article_5220.shtml} %8 2012-10-12 %X

Group 3 LEA proteins are proved to mediate plant responses to abiotic stresses such as drought, low temperature, and high salt. However, the LEA genes from common wheat (Triticum aestivum L.) have been rarely studied. We cloned a LEA gene, designated TaLEAL3, from the cDNA library of drought-treated wheat seedlings using phage hybridization in situ. The TaLEAL3 gene is 750 bp in full length and has a 501 bp open reading frame (ORF) encoding 166 amino acids. Based on multiple sequence alignment, TaLEAL3 was found to have the LEA structure characterized by α-helix and three incomplete repeat motifs comprising 11-mer amino acids. The result of electronic mapping showed that TaLEAL3 was located on chromosomes 4BL, 4DL, and 5AL. This gene was mainly expressed in stems but almost not in roots. Besides, the expression of TaLEAL3 was induced markedly by drought, low-temperature, and exogenous abscisic acid. Promoter analysis showed that the core promoter elements and cis-acting elements responding to drought and low-temperature stresses were found in the region of 1.7 kb upstream of TaLEAL3 gene. These results provided experimental data for further studying the function of LEA genes and the mechanism of LEA proteins.