%A CHENG Xin,REN De-Yong,MA Jiao,ZHU Xiao-Yan,SANG Xian-Chun,LING Ying-Hua,ZHAO Fang-Ming,HE Guang-Hua* %T Identification and Gene Mapping of Leaf Pale Yellow-Revertible Mutant pyr1 in Rice %0 Journal Article %D 2013 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2013.00992 %P 992-998 %V 39 %N 06 %U {https://zwxb.chinacrops.org/CN/abstract/article_5392.shtml} %8 2013-06-12 %X
It is important for the further understanding of the relationship between chlorophyll synthesis and degradation pathways and plant photosynthesis to identify and clone leaf color mutant gene. A leaf pale yellow-revertible mutant temporarily designated as pyr1 was obtainted from the progeny of rice (Oryza sativa L.) restorer line Jinhui 10 which was induced by ethyl methane sulfonate (EMS). In the seedling stage, the whole leaf of mutants presented pale and some mutants died. The pyr1 displayed different colors in different growth periods. At the booting stage upper-leaf and leaf margin exhibited yellow. Compared with the wild type, the chlorophyl contents of pyr1 mutant decreased from seedling stage to filling stage. Transmission electronic microscopy observation showed that the structure of cells had no obvious differences between mutant and wild type, but the chloroplast developed abnormally with degradation of the inside and matrix slices. Genetic analysis revealed that the trait was controlled by one recessive gene. With 326 recessive individuals from the F2 segregation population, the PYR1 gene was finally mapped between RM11722 and Ind1 on the long arm of chromosome 1, with an approximate physical distance of 92 kb. These results provide a basis of PYR1 gene cloning by map-based strategy.