%A TAN Qin-Liang,LI Chang-Ning,YANG Li-Tao,LI Yang-Rui %T Cloning and Expression Analysis of Abscisic Acid Signal Transduction Key Enzyme Gene SoSnRK2.1 from Sugarcane %0 Journal Article %D 2013 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2013.02162 %P 2162-2170 %V 39 %N 12 %U {https://zwxb.chinacrops.org/CN/abstract/article_5531.shtml} %8 2013-12-12 %X

Sucrose non-fermenting 1-related protein kinase (SnRK) is the key enzyme of ABA signal transduction pathways, which plays an important role in plant development under adversity environments. Reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) were applied to clone SoSnRK2.1. The cDNA full length of SoSnRK2.1 gene is 1385 bp, containing a 1002 bp complete open reading frame. The amino acids sequence analysis indicated that SoSnRK2.1 gene encodes 333 amino acids, sharing high homology with other species, especially gramineous of Zea mays and Oryza sativa. We constructed the gene prokaryotic expression vector pET-SoSnRK2.1, and obtained the protein of 38 kD induced by IPTG, which was consistent with the theoretical value. The result of Real time qPCR analysis showed that SoSnRK2.1 gene expression was basically up-regulated under different stresses of ABA, PEG+ABA, PEG, NaCl, cold, and H2O2.It indicated that SoSnRK2.1 gene takes part in regulations of drought, highsalt, low temperature or other stress process, which may play an important role in adaptation of plants to adverse stresses.