%A UN Juan,LI Wei-Xi,ZHANG Yan,SUN Li-Juan,DONG Xiao-Li,HU Xue-Xu,WANG Bu-Jun %T Simultaneous Determination of Twelve Mycotoxins in Cereals by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry %0 Journal Article %D 2014 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2014.00691 %P 691-701 %V 40 %N 04 %U {https://zwxb.chinacrops.org/CN/abstract/article_5620.shtml} %8 2014-04-12 %X

A determination method was developed using ultra-high performance liquid chromatography-tendem mass spectrometry (UPLC-MS/MS). Samples were sequentially extracted with 80% acetonitrile, and then the extracts were pooled and cleaned up with multifunction cleaning column before being separating by a chromatographic column of Waters ACQUITY UPLCTMBEH C18. The Mass spectrometry was conducted by using positive (ESI+) electrospray ionization and Multi-reactions monitoring (MRM) models, with external standard method. The results indicated that contants of the 12 mycotoxins had good linear relationship with than peak areas. The detection limits for them ranged from 0.016 to 1.000 μg kg-1, and the average recovery rates at three levels of adding external standard were from 60.0% to 122.4%, with a relative standard deviation (RSD) varying from 0.9% to 20.3%. The method is simple, rapid, accurate and suitable for simultaneously by measuring 12 kinds of mycotoxins in cereals.