%A LI Li-Na,,DU Pei,FU Liu-Yang,LIU Hua,XU Jing,QIN Li,YAN Mei,HAN Suo-Yi,HUANG Bing-Yan,DONG Wen-Zhao,TANG Feng-Shou,ZHANG Xin-You %T Development and Characterization of Amphidiploid Derived from Interspecific Cross between Cultivated Peanut and Its Wild Relative Arachis oteroi %0 Journal Article %D 2017 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2017.00133 %P 133-140 %V 43 %N 01 %U {https://zwxb.chinacrops.org/CN/abstract/article_6222.shtml} %8 2017-01-12 %X

Wild Arachis species are important genetic resources. To introgress resistant genes of Arachis species, developed a new amphidiploid AmE-4 through man-made cross between cultivated peanut variety Yuhua 9331 and a diploid Arachis species A. oteroi, with the assistance of following embryo rescue and chromosome doubling by colchicine treatment. AmE-4 was identified and characterized by fluorescence in situ hybridization (FISH) and SSR molecular marker. Morphological observation revealed significant differences in leaves between amphidiploid AmE-4 and Yuhua 9331, while the agronomic traits such as main stem height, length of first lateral branch and number of branches showed less difference between them. The date of first flower appearance in AmE-4 delayed sixty days compared with that in Yuhua 9331, and its pods setting and development were also poor, which would hinder its further utilization. In addition, 57 dominant or co-dominant SSR molecular markers were developed and could be used to identify translocation or introgression lines with A. oteroi chromosome fragment in future studies.