%A WANG Xiao-Chun,ZHANG Hao-Ran,WEI Yi-Hao,JIA Xi-Ting,GU Ming-Xin,MA Xin-Ming %T Differential Expression and Assembly Mode of Glutamine Synthetase Isoen-zymes in Different Tissues and Organs of Maize %0 Journal Article %D 2017 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2017.01410 %P 1410-1414 %V 43 %N 09 %U {https://zwxb.chinacrops.org/CN/abstract/article_6333.shtml} %8 2017-09-12 %X

Glutamine synthetase (GS) is a key enzyme in nitrogen assimilation and recycling in cereals. In this study, the expression characteristics of GS isoenzymes in different tissues and organs of maize in grain-filling period were analyzed, and the assembly of GS isoenzymes were indentified. The GS isoforms expressed differentially in different organs were shown by Western-blot obviously; GS1 with a molecular weight of about 40 kD expressed highly in all tissues, and GS1 with a molecular weight of about 39 kD was merely expressed in the node of ear position and pedical, and GS2 with a molecular weight of about 44 kD was weakly expressed in the photosynthtic tissue such as leaf. With a modified blue naive PAGE (BNE) technique and in-gel activity analysis, the size of GS holoenzyme was calibrated; combined the 2-D gel with western-blot analysis, the subunits composition of GS isoenzymes were identified. Three GS isoenzymes with different sizes were identified in maize. GS2 holoenzyme was about 460 kD and likely a decamer, GS1 holoenzyme existed two kinds of assembly state, one was about 410 kD and likely a decamer, another was about 240 kD and more likely a pentamer; therefore, the expression of GS isoenzymes exists diversity in maize.