%A DO Thanh-Trung, LI Jian, ZHANG Feng-Juan, YANG Li-Tao, LI Yang-Rui,XING Yong-Xiu %T Analysis of Differential Proteome in Relation to Drought Resistance in Sugarcane %0 Journal Article %D 2017 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2017.01337 %P 1337-1346 %V 43 %N 09 %U {https://zwxb.chinacrops.org/CN/abstract/article_6352.shtml} %8 2017-09-12 %X

Drought stress is a major restraint in sugarcane production in China. Proteomic study in relation to drought stress provides valuable information in drought resistant breeding of sugarcane. In this study, the drought-resistant sugarcane variety, F172, and the drought-sensitive variety, YL6, were used in a pot experiment for differential proteome analysis. Seedlings of both varieties were exposed to severe drought stress for seven days and the leaf proteins were separated and analyzed using 2-DE technique and PDQuest software. From the protein profiles of F172 and YL6, 28 and 20 differential protein spots were detected between normal-irrigation and drought-stress treatments, respectively, including up- and down-regulated proteins and new protein spots. The differential proteins varied across the two varieties. Using MALDI-TOF-TOF/MS, 18 and 14 amino acid sequences were identified from YL6 and F172, respectively, and they were in eight function categories. In YL6, the 18 proteins consist of two participating in oxygen radical scavenging, six participating in photosynthesis, one participating in cell growth and division, six participating in basic metabolisms, two participating in protective response, and one unknown in function. In F172, the 14 proteins consist of one participating in oxygen radical scavenging, two participating in photosynthesis, two participating in cell growth and division, four participating in basic metabolisms, two participating in information transfer, one participating in protein processing, and one unknown in function. A drought-induced protein of 22 kDa was in high level in F172 but absence in YL6. These results indicate that protein compositions under drought stress are highly different in sugarcane varieties with different drought resistance and the differential proteins might give a hint to drought-resistant mechanism.