%A Chao LI,Zhi-Kun LI,Qi-Shen GU,Jun YANG,Hui-Feng KE,Li-Qiang WU,Guo-Ning WANG,Yan ZHANG,Jin-Hua WU,Gui-Yin ZHANG,Yuan-Yuan YAN,Zhi-Ying MA,Xing-Fen WANG %T Molecular Evaluation for Chromosome Segment Substitution Lines of Gossypium barbadense and QTL Mapping for Fiber Quality and Yield %0 Journal Article %D 2018 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2018.01114 %P 1114-1126 %V 44 %N 8 %U {https://zwxb.chinacrops.org/CN/abstract/article_6528.shtml} %8 2018-08-10 %X

In the previous study, we developed a set of chromosome segment substitution lines (CSSLs) using G. hirsutum CCRI8 as the recipient parent and G. barbadense Pima 90-53 as the donor parent. In this study, we genotyped the BC3F5 generation of CSSLs with SSR markers, conducted QTL mapping for the fiber quality and yield traits and identified the stable QTLs in three different environments (Baoding, Qingxian, Luntai). The substituted segment number of the 182 CSSLs varied from one to fifteen, averaged 6.6. The length of introgressed segments ranged from 0.7 cM to 83.2 cM, and averaged 16.8 cM. The total length of the substituted fragment was 20 249.6 cM, background recoverage rate varied from 92.3% to 99.6%, and the average background recoverage rate was 96.2%. Fifty-nine QTLs related to fiber quality and yield traits were detected. Among them, 41 QTLs were related to fiber quality traits and each QTL explained 1.27% to 26.66% of the phenotypic variation. Eighteen QTLs for fiber yield-traits including boll weight and lint percentage were detected and each QTL explained 2.03% to 19.38% of the phenotypic variation. Fourteen stable QTLs were detected in multiple environments. Among them, four QTLs related to micronaire value and two QTLs related to fiber elongation both had enhancing alleles from G. barbadense Pima 90-53. Two boll weight QTLs had enhancing alleles from G. hirsutum CCRI8. The results provide a theoretical basis for QTL fine mapping, QTL interaction and molecular breeding for fiber quality and yield traits.