%A Zhong-Xiang LIU,Mei YANG,Peng-Cheng YIN,Yu-Qian ZHOU,Hai-Jun HE,Fa-Zhan QIU %T Fine Mapping and Genetic Effect Analysis of a Major QTL qPH3.2 Associated with Plant Height in Maize (Zea mays L.) %0 Journal Article %D 2018 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2018.01357 %P 1357-1366 %V 44 %N 9 %U {https://zwxb.chinacrops.org/CN/abstract/article_6544.shtml} %8 2018-09-10 %X

Plant height is one of the most important factors affecting maize yield, which is determined by the number and the length of internode in maize. In this research, two advanced-backcross recombinant inbred lines (W1 and W2) with significant difference in plant height were used. They have the same number of internodes. We found that the different cell lengths of the internode in upper spike were the main reason causing the difference in plant height. The results of exogenous GA test showed that the QTL/genes controlling plant height were not included in GA pathway. The F2 and F2:3 populations derived from W1 and W2 were used to map the QTLs associated with plant height, showing that one major named qPH3.2 was commonly identified under three different environments in two years which was located on chromosome 3 between markers C42 and P17 with 20 Mb and could explain 22.22% of phenotypic variation. On the basis of the primary mapping results, QTL qPH3.2 was divided into two major QTLs qPH3.2.1 and qPH3.2.2 via recombinant exchange individuals and its self-cross progeny. Furthermore, we did the fine mapping work for qPH3.2.1 and qPH3.2.2 using substitution lines. The qPH3.2.1 was fine-mapped to the region of about 2 Mb between markers YH305 and Y72, and qPH3.2.2 was fine-mapped to the region of about 1.6 Mb between markers YH112 and Y150, which all showed the positive additive effects. The results of this research provide reliable genetic loci for the genetic improvement of plant height in maize, and a good foundation for cloning QTLs for plant height in the future.