%A Lin-Sheng WANG,Ya-Li ZHANG,Guang-Hui NAN %T Molecular and Cytogenetic Identification of Triticum aestivum-Leymus racemosus Translocation Line T5AS-7LrL·7LrS %0 Journal Article %D 2018 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2018.01442 %P 1442-1447 %V 44 %N 10 %U {https://zwxb.chinacrops.org/CN/abstract/article_6551.shtml} %8 2018-10-10 %X
Leymus racemosus is highly resistant to wheat scab. The transfer of resistance genes from L. racemosus to common wheat (Triticum aestivum) is important for broadening the resistant sources against scab in common wheat. In this study, the pollen of DA7Lr, a T. aestivum-L. racemosus disomic addition line with scab resistance, was irradiated with 60Co-γ ray at 1200 Rad (100 Rad min -1) before pollinating to emasculated T. aestivum cv. Chinese Spring. One plant with one translocation chromosome was detected in the M1 generation by GISH. This plant was then self-pollinated and the pollen mother cells (PMCs) of the offspring plants with two translocation chromosomes were cytologically observed, and one ring bivalent was found at meiotic metaphase I, indicating that the plant with two translocation chromosomes was one translocation homozygote. The translocation line was proved to be T5AS-7LrL·7LrS by C-banding, and sequential GISH-FISH using Oligo-pAs1-2 and Oligo-pSc119.2-2 as probes. Three EST-STS markers (BE591127, BQ168298, and BE591737) were identified to be able to track the T5AS-7LrL·7LrS line. The translocation line also serves as an resistant source against wheat scab in wheat breeding programs.