%A Jing DONG,Xiao-Ping LU,Kun-Ming ZHANG,Chun-Lei XUE,Rui-Xia ZHANG %T Analysis of SNP and Allele-specific Expression in Transcriptome of Sorghum bicolor × Sorghum sudanense and Their Parents %0 Journal Article %D 2018 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2018.01809 %P 1809-1817 %V 44 %N 12 %U {https://zwxb.chinacrops.org/CN/abstract/article_6580.shtml} %8 2018-12-12 %X

Taking root, stem and leaf tissues of S. sudanense hybrids and their parents as test materials, Illumina Hiseq 2000 was used to analyze the transcriptome to explore the relationship between single nucleotide variation and heterosis in the hybrids of Sorghum bicolor × S. sudanense and their parents. About 58 000 SNP loci were detected from the sequencing samples with an average length of 58 122 160 bp. The number of genic SNP was significantly more than that of intergenic SNP. The frequency of SNP was 1/741 bp, and the conversion ratio of the average conversion was 1.00:1.53. Among all the types of variation, C/T and G/A had the highest frequency. After screening, 198 (21%) extremely significant biased alleles were expressed in bias SNP, and 65% of them were biased towards paternal white shell S. sudanense, and many of the transcriptional copies with high level gene expression in the white shell S. sudanense were also expressed in the Sorghum bicolorss × S. sudanense hybrid. The two parental alleles with 79%, 78%, and 82% transcripts showed a stable level of expression in the three tissues. It is suggested that the trans-acting may affect the specific expression of the allele more than the cis-acting. Six highly-biased SNP-unigene alleles were selected for qRT-PCR validation. The differential gene expression pattern of these genes was consistent with that of RNA-Seq analysis. Illumina sequencing technology was used to study allelic expression in this study, provides a basis for heterosis analysis of Sorghum bicolor × S. sudanense and also a theoretical reference for related studies of other forage crops.