%A Tao FENG,Chun-Yun GUAN %T Cloning and Characterization of Brassinazole-resistant (BnaBZR1 and BnaBES1) CDS from Brassica napus L. %0 Journal Article %D 2018 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2018.01793 %P 1793-1801 %V 44 %N 12 %U {https://zwxb.chinacrops.org/CN/abstract/article_6588.shtml} %8 2018-12-12 %X

Brassinazole-resistant (BZR) is a key transcription factor in brassinosteroid signaling pathway of plants, containing brassinazole-resistant 1 (BZR1) and brassinazole-resistant 2 (BES1). In this study, three novel BZR1 coding sequences (CDSs) were isolated from cDNA of Brassica napus L. cv. Xiangyou 15 leaves, which were mapped on the chromosomes A07, A06, and A06, and designated as BnaBZR1_A07, BnaBES1_A06F, and BnaBES1_A06R, respectively. These three BnaBZR CDSs were 996, 993, and 996 bp in length and encoded predicted proteins with 331, 330, and 331 amino acid residues, respectively. BnaBZR proteins were predicted to be located on the cell nucleus and have a typical plant BZR/BES conserved domain. Multiple sequence alignments and phylogenetic analysis showed that the deduced amino acid sequences of BnaBZR were highly homologous to previously reported BZR/BES of Brassica oleracea, Arabidopsis thalian, and Eruca sativa. And the similarity of BZR1 or BES1 among different related species was higher than the similarity between BZR1 and BES1 in the same species or related species, indicating that BZR1 and BES1 differentiation is an early evolutionary event. The expression patterns of BnaBZR1_A07, BnaBES1_A06F, and BnaBES1_A06R in Xiangyou 15 were similar, whit high expression level at the seedling stage and flowering stage, while slightly lower level at the stage of bolting and podgrain ripening. The expression level of BnaBZR in the aerial parts such as leaves, stems, flowers and pods was higher than that in underground parts.