%A Xiao-Ding MA,Jiang-Hong TANG,Jia-Ni ZHANG,Di CUI,Hui LI,Mao-Mao LI,Long-Zhi HAN %T Development of molecular markers polymorphic between Dongxiang wild rice and Geng rice cultivar ‘Nipponbare’ %0 Journal Article %D 2019 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2019.82037 %P 316-321 %V 45 %N 2 %U {https://zwxb.chinacrops.org/CN/abstract/article_6628.shtml} %8 2019-02-12 %X

Dongxiang wild rice is a type of common wild rice (Oryza rufipogon) that has the northernmost natural distribution of any wild rice species. The genome of Dongxiang wild rice differs from that of modern cultivated rice varieties (Oryza sativa). At present, a set of molecular markers that covers the entire genome of Dongxiang wild rice is lacking. In this study, we used Dongxiang wild rice and the Geng rice variety “Nipponbare” as research materials. By screening the existing collection of 1017 SSR and InDel markers and the 217 InDel markers which were designed using Dongxiang wild rice genome resequencing information, we obtained a set of 203 markers polymorphic between Dongxiang wild rice and ‘Nipponbare’, which were relatively uniformly distributed on the 12 rice chromosomes, and basically covered the entire genome, with an average inter-locus distance of 1.9 Mb. Through the genotyping of five Xian and five Geng varieties, we concluded that those 203 polymorphic molecular markers have a high application value in genotype analysis of Dongxiang wild rice and Geng rice offspring population. These results provide a powerful tool for exploring beneficial genes from Dongxiang wild rice as well as marker-assisted breeding and selection.