%A YE Wei-Jun,CHEN Sheng-Nan,YANG Yong,ZHANG Li-Ya,TIAN Dong-Feng,ZHANG Lei,ZHOU Bin %T Development of SSR markers and genetic diversity analysis in mung bean %0 Journal Article %D 2019 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2019.84155 %P 1176-1188 %V 45 %N 8 %U {https://zwxb.chinacrops.org/CN/abstract/article_6710.shtml} %8 2019-08-12 %X

SSR markers play an important role in basic research and crop breeding due to their advantages of large number, high polymorphism and co-dominant inheritance. However, there are still few SSR markers available in mung bean. In this study, the magnetic bead enrichment method and sequencing technology were combined to identify the SSR loci of mung bean in high throughput, a total of 3,275,355 SSR loci were found, and 2742 markers were developed. A total of 157 markers were selected for validation by PCR method, 90 (57.33%) showed polymorphic among 10 mung bean accessions. Forty SSR markers with clear PCR products, high polymorphism and uniform distribution on chromosomes were selected to evaluate the genetic diversity among 90 mung bean accessions. The number of alleles per marker varied from two to eight, with an average of three. The effective number of alleles ranged from 1.31 to 4.21, with a mean value of 2.16. The Nei’s gene diversity was between 0.23 and 0.76, with an average of 0.51. Polymorphism information content was between 0.22 and 0.72, with a mean of 0.43. Cluster analysis distributed 90 materials into two clusters, including four groups. The germplasm of group II came from several areas, while those of groups I and III were mainly from North China and Shandong province, respectively. Most of the gerplasm from Hebei province were clustered in Group IV. These polymorphic SSR markers will be valuable for genetic diversity analysis, high-resolution genetic linkage maps construction, gene mapping and marker assisted selection in mung bean breeding.