%A GAO Huan-Huan,YE Sang,WANG Qian,WANG Liu-Yan,WANG Rui-Li,CHEN Liu-Yi,TANG Zhang-Lin,LI Jia-Na,ZHOU Qing-Yuan,CUI Cui %T Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus %0 Journal Article %D 2019 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2019.84169 %P 1416-1430 %V 45 %N 9 %U {https://zwxb.chinacrops.org/CN/abstract/article_6730.shtml} %8 2019-09-12 %X

Seed germination is an important stage of plant morphogenesis in rapeseed. Aluminum toxicity is one of the main factors affecting seed germination in acidic soil. Therefore, it is important for rapeseed production and research to screen Al-tolerant germplasms during seed germination. In this study, five Brassica napus cultivars (lines) were used to screen the optimum treatment concentration of Al toxicity at germination stage, which was determined at 90 μg mL -1. The germination vigor, germination rate, fresh weight, dry weight, root length and bud length of 148 cultivars (lines) of Brassica napus were measured at germination stage. The comprehensive evaluation value (A value), average subordinate function value (ASF value), weighted tolerance coefficient (WAC value), correlation coefficient, frequency, principal component, grey relational degree, clustering and stepwise regression were used to identify the Al tolerance at germination stage. A comprehensive evaluation model of Al tolerance at germination stage was established, suitable evaluation and identification indexes were selected. There were significant differences in the germination parameters of 148 Brassica napus germplasm, and aluminum-tolerant Brassica napus varieties (lines) during germination stage were screened, including 01188, WH-20, A109, and Jiayu 31 peng. The results of grey correlation and regression analysis indicated that the root length, bud length, fresh weight, germination rate and germination potential measured at the germination stage of rapeseed, and the A value of the indices estimated by regression equation, could be preliminarily judged the aluminum tolerance of rape germplasm.