%A CHEN Xiao-Jing,LIU Jing-Hui,YANG Yan-Ming,ZHAO Zhou,XU Zhong-Shan,HAI Xia,HAN Yu-Ting %T Effects of salt stress on physiological indexes and differential proteomics of oat leaf %0 Journal Article %D 2019 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2019.81088 %P 1431-1439 %V 45 %N 9 %U {https://zwxb.chinacrops.org/CN/abstract/article_6734.shtml} %8 2019-09-12 %X

MDA content, SOD and POD activities, free proline content of oat leaves were determined under salt stress (molar NaCl: Na2SO4=1:1), and the differentially expressed proteins in leaves were analyzed by using Label-Free technique. The results showed that the activities of MDA, SOD and POD in oat leaves decreased by 16.7%, 23.4%, and 21.2% respectively, and free proline content increased by 1.12% compared with the control. There were 76 differential proteins with P-value ≤ 0.05 and ratio > 2 (51 proteins up-regulated and 25 proteins down-regulated). The GO annotation indicated that 27 differential proteins were significantly enriched in 16 metabolic pathways, of which the oxidation-reduction process was 33.9%. The biological processes of level 3 statistical enrichment were oxygen binding and oxidoreductase activity. Twenty-two differential proteins were obtained using KEGG annotation, which was significantly enriched in 10 biochemical metabolic pathways, mainly including four processes: protein processing in endoplasmic reticulum, longevity regulating pathway-multiple species, antigen processing and presentation, estrogen signaling pathway. The STRING protein interaction network showed that 10 of the 21 differential proteins involved in post-translational modification, protein turnover, and molecular chaperone function, and HSP70 and HSP90 interacted with the most core proteins of the whole network. It is speculated that the up-regulation of core protein is one of the reasons for the salt tolerance of oats.