%A XU Ting-Ting, WANG Qiao-Ling, ZOU Shu-Qiong, DI Jia-Chun, YANG Xin, ZHU Yin, ZHAO Han, YAN Wei %T Development and application of InDel markers based on high throughput sequencing in barley %0 Journal Article %D 2020 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2020.91076 %P 1340-1350 %V 46 %N 9 %U {https://zwxb.chinacrops.org/CN/abstract/article_6954.shtml} %8 2020-09-12 %X

Molecular markers are the basic tools in genetic research and widely used in genetic diversity analysis, germplasm identification, genetic map construction and gene mapping. Based on the whole genome re-sequencing data of barley, we developed 118 pairs of InDel markers covering the whole barley genome. Forty-nine barley germplasms from different geographical regions were used to detect the effectiveness of primers. And 72 co-dominant InDel markers with two alleles were selected for further genetic distance analysis in 288 barley germplasm and constructing phylogenetic tree. Thirty-two core InDel markers were screened, covering seven chromosomes of barley, with an average PIC of 0.44, and an average MAF of 0.34. The tested barley germplasms were rich in genetic diversity and could cluster most of the varieties with the same geographical origin. This study indicated that the 32 dimorphic InDel markers developed not only effectively reflect the genetic relationship between varieties, but also enrich the molecular markers used for the identification of barley varieties. The above core InDel markers have provided certain the theoretical significance and application value in on the identification of barley varieties, the relationship analysis of barley resources and group division of barley resources.