%A GUO Qing-Yun, KUAI Jie, WANG Bo, LIU Fang, ZHANG Chun-Yu, LI Gen-Ze, ZHANG Yun-Yun, FU Ting-Dong, ZHOU Guang-Sheng %T Effect of mixed-sowing of near-isogenic lines on the clubroot disease controlling efficiency in rapeseed %0 Journal Article %D 2020 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2020.04074 %P 1408-1415 %V 46 %N 9 %U {https://zwxb.chinacrops.org/CN/abstract/article_6978.shtml} %8 2020-09-12 %X

The clubroot disease caused by Plasmodiophora brassicae has become a serious threat to rapeseed production in these recent years. In the previous multi-plot and multi-season field experiments, we found that planting of susceptible and resistant cultivars (near-isogenic lines) at different proportions could significantly suppress the incidence of the clubroot disease. In order to explore the preventive and control effect of mix-sowing of susceptible and resistant cultivars on clubroot disease, and identify the factors affecting mixed sowing. Pot experiments with different distance (0, 2, 4, and 6 cm) between the seeds of the resistant and susceptible cultivars were set by artificial inoculation at the 7 days after germination in this study. The disease incidence, disease index and the root biochemical components of rapeseed were measured after forty-two days. The results were as below: (1) the incidence of the resistant cultivar showed no significant change, while the disease incidence and disease index of the susceptible rapeseed cultivars decreased in the mixed-sowing treatment. Further, the disease incidence was significantly influenced by sowing distance of the two cultivars, which was significantly (P < 0.05) lower than those of the other treatments when mixed-sowed at 0 cm. (2) compared to the single planting, the content of soluble sugar and soluble protein in roots of the mixed-sowing treatment were significantly (P < 0.05) decreased for the two cultivars, and the content of acid-insoluble lignin was significantly increased in susceptible cultivar whereas decreased after increasing in resistant cultivar. (3) the results of correlation analysis showed that the content of soluble sugar and soluble protein were positively correlated with the disease incidence and disease index. The correlation coefficients between disease incidence and soluble sugar and soluble protein content were 0.797 and 0.403, respectively, meanwhile, the correlation coefficients between disease index and soluble sugar and soluble protein content were 0.822 and 0.509, respectively. In conclusion, the findings provided a new idea for prevention of clubroot disease in the field production in rapeseed.