%A WU Hai-Tao, ZHANG Yong, SU Bo-Hong, Lamlom F Sobhi, QIU Li-Juan %T Development of molecular markers and fine mapping of qBN-18 locus related to branch number in soybean (Glycine max L.) %0 Journal Article %D 2020 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2020.04043 %P 1667-1677 %V 46 %N 11 %U {https://zwxb.chinacrops.org/CN/abstract/article_6985.shtml} %8 2020-11-12 %X

The branch number is one of the important factors influencing soybean yield, which is directly related to pod setting rate. At the same time, it is also an important component of soybean plant type, and further affects the yield by adjusting the population structure and planting density. At present, there is few report related to map-based cloning of genes related to branch number. Therefore, the discovery of genes/QTL involved in the regulation of soybean branching is of great significance for the basic research on the establishment of plant type and the applied research on the development of high-yielding varieties. In this study, based on the F2 of crossing low-branched variety Kenfeng 19 (KF19) and high-branched variety Kennong 24 (KN24), we developed the F7:8 recombinant inbred line (RIL) population, consisting of 606 lines, and two backcrossing populations consisting of 1486 individuals for KF19-BC3F2 and 1150 individuals for KN24-BC2F2. Within the localization interval of the new QTL of the branch number of chromosome 18 (qBN-18), 11 polymorphism SSR markers were screened out to identify the RIL population, and region of qBN-18 was reduced from 1.6 Mb to 113 kb. After developing two InDel markers BR69 and BR77 in the mapping region, the backcross population was used to screen the exchange individuals, the interval of qBN-18 was further reduced to 63.7 kb, including 9 genes. Those results provide the information for gene map-based cloning and molecular marker assisted breeding of branch number in soybean.