%A WANG Yan-Hua, JIAN Hong-Jiu, QIU Xiao, LI Jia-Na %T Regulatory mechanism of the seed coat color gene BrTT1 in Brassica rapa L. %0 Journal Article %D 2020 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2020.04036 %P 1678-1689 %V 46 %N 11 %U {https://zwxb.chinacrops.org/CN/abstract/article_6986.shtml} %8 2020-11-12 %X

Brassica rapa (B. rapa L., 2n = 20, AA) is a specie of Brassica genus, belonging to the basic species of cultivated rapeseed. China is the original center of Chinese cabbage and Brassica campestris. Compared with Brassica napus, it has a long history of origin and cultivation and rich genetic resources, which has natural and stable yellow seed resources. Dahuang has the natural yellow seed resource in B. rapa. Its seed coat color is bright yellow, the yellow seed trait can be stably inherited, and Dahuang has the advantages of large grain, high oil content and good self-adhesiveness. Sequence comparison showed that nucleotide polymorphisms were solely found in BrTT1 sequences from different seed color lines (yellow, red or brown, and black), which could be used to predict seed color phenotype. Yeast two-hybrid analysis indicated BrTT1 could interact with two other transcriptional factors R2R3-MYB (BrTT2) and WD40 (BrTTG1), and one catalytic enzyme (BrTT3). Quantitative RT-PCR analysis of transgenic B. rapa lines with the gene down-regulated by RNA interference and up-regulated by overexpression revealed that two contrasting groups of genes were regulated by BrTT1 in the biosynthesis and deposition of flavonoids pigments in the seed of B. rapa. These results further define the regulatory activity of BrTT1 in seed coat color formation in Brassica species.