%A JIANG Hong-Rui, YE Ya-Feng, HE Dan, REN Yan, YANG Yang, XIE Jian, CHENG Wei-Min, TAO Liang-Zhi, ZHOU Li-Bin, WU Yue-Jin, LIU Bin-Mei %T Identification and gene localization of a novel rice brittle culm mutant bc17 %0 Journal Article %D 2021 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2021.02025 %P 71-79 %V 47 %N 1 %U {https://zwxb.chinacrops.org/CN/abstract/article_7022.shtml} %8 2021-01-12 %X

A brim culm mutant bc17 (brittle culm 17) was obtained by irradiating wyj7 (Wuyunjing 7) with heavy ions. The brittle traits of the mutant were only found in the stalks and not in the leaves. The brittleness of the culm began to appear after heading stage, while it became more obvious as rice grew from heading stage to maturity stage. The growth and development of the mutant were affected, the plant height of the mutant was significantly lower than that in the wild type, and tiller number and seed setting rate were also lower than in the wild type. Compared with wild type, the cellulose content in bc17 culms and leaves decreased by 22.7% and 18.67%, while the hemicellulose content increased by 45.76% and 31.36%, respectively. The breaking resistance and tensile force of bc17 were significantly lower than those of wild type, indicating that the mechanical strength of the culm changed. The thick-walled cells of bc17 culms had larger pores, looser structures, and fewer cells. The fragile characteristics of bc17 were controlled by a single recessive nuclear gene. The bc17 gene was located in the 162 kb region of chromosome 7 by map-based cloning. Bioinformatics analysis indicated that it might be a novel gene related to rice brittle culm. These findings provided an important material support for the research on the molecular mechanism of cell wall synthesis in rice.