%A WANG Heng-Bo, CHEN Shu-Qi, GUO Jin-Long, QUE You-Xiong %T Molecular detection of G1 marker for orange rust resistance and analysis of candidate resistance WAK gene in sugarcane %0 Journal Article %D 2021 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2021.04131 %P 577-586 %V 47 %N 4 %U {https://zwxb.chinacrops.org/CN/abstract/article_7070.shtml} %8 2021-04-12 %X

Sugarcane orange rust is an important fungal disease caused by Puccinia kuehnii Butler, which could lead to a reduction in sugarcane production and sugar content and cause serious losses to the sugarcane industry in worldwide. In this study, the molecular marker G1 was used to detect orange rust resistance genes in cultivars, ancestral species and the related genus of Saccharum in the world. The representative amplified bands were cloned, sequenced, functionally annotated, and clustered, and the origin and evolution of resistance genes was then analyzed. The results showed that 83 and 34 were detected with G1 marker, accounting for 66.9% and 67.4% in 124 Chinese and 46 foreign sugarcane cultivars, respectively. Among 34 sugarcane ancestral species and the related genus of Saccharum, 17 were detected by G1 marker, accounting for 50%, of which the highest percentage (100%) was in Saccharum spontaneum. Functional annotation revealed that G1 target gene encoded a wall-associated receptor-like kinase (WAK), and three proteins with high similarity were identified from the haploid proteome database of sugarcane cultivars. These proteins all contain the extracellular domain, transmembrane domain and intracellular domain with kinase activity of typical cell wall receptor structures. Phylogenetic analysis of nucleotide sequences clearly showed the origin and evolution of the candidate resistance WAK genes. Specifically, the WAK genes amplified by G1 marker could be divided into three groups. The first group is from S. spontaneum and Saccharum robustum. The second group is from S. robustum, Saccharum officinarum and Narenga porphyrooma. The third group is from S. spontaneum, S. robustum, Saccharum sinense and cultivars. These results provided an important support for breeding of sugarcane cultivars resistant to orange rust, and lay a foundation for further analysis of molecular mechanism of resistance genes.