%A HUANG Wen-Gong, JIANG Wei-Dong, YAO Yu-Bo, SONG Xi-Xia, LIU Yan, CHEN Si, ZHAO Dong-Sheng, WU Guang-Wen, YUAN Hong-Mei, REN Chuan-Ying, SUN Zhong-Yi, WU Jian-Zhong, KANG Qing-Hua %T Transcriptome profiling of flax (Linum usttatissimum L.) response to low potassium stress %0 Journal Article %D 2021 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2021.04133 %P 1070-1081 %V 47 %N 6 %U {https://zwxb.chinacrops.org/CN/abstract/article_7114.shtml} %8 2021-06-12 %X

Potassium (K) is an essential element for the growth and development in flax. Transcriptome sequencing and qRT-PCR were used to investigate the regulation of differential gene expression after 12 h and 96 h of low-K + treatment. The results showed that the leaf edge of flax treated with low-K + for 7 days turned yellow, and the plants were dwarfed compared with the control. LusKC1 (Lus K channel 1), LusSKOR (Lus STELAR K + outward rectifier) and LusHAK5 (Lus high affinity K + transporter 5) were detected to respond to low-K + with response peak time of 12 h and 96 h. Compared with the control, 1154 differentially expressed genes (DEGs) (508 up-regulated and 646 down-regulated genes) were identified in low-K + treatment for 12 h. GO enrichment showed that DEGs were mainly concentrated on five categories: metabolic process, cellular process, single biological process, catalytic activity and binding function. KEGG pathway enrichment showed that DEGs involved in energy metabolism, carbohydrate metabolism, carbon metabolism, amino acid metabolism, terpenoid metabolism and plant hormone signal transduction pathways. Furthermore, 7 genes directly related to K (4K transporters, 2K channel proteins and 1 sodium-potassium-calcium exchanger protein), 13 genes related to hormone and 6 genes related to cellulose synthesis were screened. Among the 7 genes directly related to K, the relative expression of 2 genes were up-regulated by 1.75 and 2.64 times and 5 genes down-regulated by 1.21-9.57 times. In summary, DEGs preliminarily revealed the transcriptional regulation pathway involved in low-K + in flax, which laid a foundation for cloning and functional verification of flax low-K + tolerance related genes.