%A GENG La, HUANG Ye-Chang, LI Meng-Di, XIE Shang-Geng, YE Ling-Zhen, ZHANG Guo-Ping %T Genome-wide association study of β-glucan content in barley grains %0 Journal Article %D 2021 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2021.01074 %P 1205-1214 %V 47 %N 7 %U {https://zwxb.chinacrops.org/CN/abstract/article_7126.shtml} %8 2021-07-12 %X

β-glucan is an important trait in barley, as its content greatly affects the quality in the applications of malting, feeding, and food. Although the genes associated with β-glucan synthesis have been reported, genetic regulation of β-glucan accumulation in barley grains is still unclear. In this study, genome-wide association study (GWAS) with mixed linear model (MLM) and general linear model (GLM) was performed to analyze the grain β-glucan content of 119 barley germplasms collected from worldwide previously, which were planted at two plots with certain differences in soil and climate conditions. The results showed β-glucan content in barley grains was significantly different in genotypes and the heritability of β-glucan was 73.9% in two environments. There were eight and 40 loci for grain β-glucan content detected by MLM and GLM, respectively. A total of 44 loci were obtained by combining the same loci of the two models. HORVU5Hr1G022710 gene identified in both models and sites was considered as a putative candidate gene significantly associated with β-glucan content. Significantly positive correlation was detected between grain β-glucan content and the number of favorable alleles in both models. In addition, 10 enzymatic genes related to sugar synthesis, transport and decomposition were identified based on gene annotations. These genes may significantly relate to β-glucan synthesis, accumulation and hydrolysis. The results provided a new insight into the genetic regulation of β-glucan accumulation and laid a foundation for the genetic improvement breeding of barley seed β-glucan.