%A LI Jie, FU Hui, YAO Xiao-Hua, WU Kun-Lun %T Differentially expressed protein analysis of different drought tolerance hulless barley leaves %0 Journal Article %D 2021 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2021.01062 %P 1248-1258 %V 47 %N 7 %U {https://zwxb.chinacrops.org/CN/abstract/article_7131.shtml} %8 2021-07-12 %X

In order to reveal the differences in response to drought stress among different hulless barley varieties from the protein level and to analyze the protein molecular mechanism of drought tolerance, Handizi Barley (HDZ) resistant to drought stress and Dama Barley (DM) sensitive to drought stress were used as research materials in this study. Drought treatment were determined by potted-planting method with limited water supply, four physiological indexes of hulless barley leaves with different drought gradients, including chlorophyll, soluble protein, malondialdehyde content, and relative conductivity were investigated. iTRAQ technology was used to conduct differential protein analysis on the whole protein group of barley leaves under deep drought stress. The results showed that with the extension of the drought treatment, the chlorophyll and soluble protein content of two hulless barleys under drought stress gradually decreased, the electrical conductivity and malondialdehyde content gradually increased, and the decrease in chlorophyll and soluble protein content, the increase in electrical conductivity and the content of malondialdehyde in Dama were greater than that of Handizi; 4163 proteins (polypeptides) were quantified, among them, compared with normal culture in the Handizi comparison group, 68 up-regulated proteins and 63 down-regulated proteins were screened by iTRAQ; in the comparative group of Dama, 21 up-regulated proteins and 32 down-regulated proteins were screened. KEGG pathway showed that the top three enrichment pathways were metabolic, amino acid biosynthesis, and secondary metabolite biosynthesis. The first one mainly related to citric acid cycle, carbon cycle, and other metabolic pathways. The synthesis and degradation of amino acids were mainly involved arginine and alanine. The synthesis of secondary metabolites were about arachidonic acid and linolenic acid. This study screened the proteins related to the metabolic pathways and other related functions in response to drought stress on proteome level in hulless barley, providing a theoretical basis for revealing the molecular regulation mechanism in response to drought stress.