%A XUE Xiao-Meng, WU JIE, WANG Xin, BAI Dong-Mei, HU Mei-Ling, YAN Li-Ying, CHEN Yu-Ning, KANG Yan-Ping, WANG Zhi-Hui, HUAI Dong-Xin, LEI Yong, LIAO Bo-Shou %T Effects of cold stress on germination in peanut cultivars with normal and high content of oleic acid %0 Journal Article %D 2021 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2021.04170 %P 1768-1778 %V 47 %N 9 %U {https://zwxb.chinacrops.org/CN/abstract/article_7172.shtml} %8 2021-09-12 %X

High oleate (HO) peanut is highly popular for its improved nutrient value and strengthened storage stability among customers and peanut processing enterprises. In recent years, with the adoption of HO peanut in our country, the cold tolerance of peanut at germination stage in high altitude or high latitude area has become a major concern. To figure out the relationship between the seed oleic acid content and the cold tolerance at germination stage in peanut, the germination rate and germination index of seeds under cold stress were investigated among six peanut cultivars with normal content of oleic acid (NO) and their backcross-derived HO lines, respectively. The results showed that the oleic acid content was not significantly correlated with the cold tolerance at germination stage. The contents of eight main fatty acids under cold stress at germination stage were tracked in Quanhua 551 (Quanhua 551-NO) and its HO offspring line (Quanhua 551-HO), and the germination rate of Quanhua 551-HO was significantly lower than that of Quanhua 551-NO under cold stress. The oleic acid content of Quanhua 551-NO was significantly decreased while the linoleic acid content was significantly increased under cold stress. However, the contents of oleic acid and linoleic acid exhibited the same trend in Quanhua 551-HO, and there was no significant difference. The expression profiles offatty acid desaturase 2 (AhFAD2) genes in both Quanhua 551-NO and Quanhua 551-HO under cold stress revealed that the relative expression level of AhFAD2-1A/B was significantly up-regulated, while that of AhFAD2-4A/B was significantly down-regulated under cold stress in Quanhua 551-NO. Conversely, the relative expression level of AhFAD2-1A/B was significantly decreased, but the relative expression level of AhFAD2-4A/B was significantly increased under cold stress in Quanhua 551-HO. These results implied that the up-regulation of AhFAD2-4A/Bin HO peanut may partly compensate for lost function of AhFAD2-1A/Bin response to cold stress. In conclusion, the oleic acid content in seed was not the main factor to determinate the cold tolerance at germination stage.