%A LU Hai, LI Zeng-Qiang, TANG Mei-Qiong, LUO Deng-Jie, CAO Shan, YUE Jiao, HU Ya-Li, HUANG Zhen, CHEN Tao, CHEN Peng %T DNA methylation in response to cadmium stress and expression of different methylated genes in kenaf %0 Journal Article %D 2021 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2021.04269 %P 2324-2334 %V 47 %N 12 %U {https://zwxb.chinacrops.org/CN/abstract/article_7222.shtml} %8 2021-12-12 %X

DNA methylation is one of the important epigenetic modifications in plants and plays an important role in response to stress. However, there are few reports of the changes of DNA methylation levels in plants under cadmium stress. The seedlings of kenaf P3A were treated with 300 μmol L-1 CdCl2 by hydroponics, the agronomic traits and cadmium contents were investigated. The changes of DNA methylation level of root under cadmium stress were analyzed by using methylation sensitive amplified polymorphism (MSAP) method. In addition, the differential methylation fragments were recovered, cloned, and sequenced. The relative expression levels of differential methylated genes revealed that Cd stress significantly inhibited the plant height, stem diameter, root length, root surface area, and total fresh weight of kenaf seedlings. The whole DNA methylation ratio in root was 68.23% and 62.78% under cadmium and control treatments, respectively. Among them the total methylation ratio was 37.50% and 36.36%, and the semi-methylation ratio was 25.28% and 31.87%, respectively. These results indicated that cadmium stress significantly increased the DNA methylation level of roots DNA. Seven differential methylated genes which involved in stress resistance were characterized differentially expressed under cadmium stress, suggesting that the change of DNA methylation played an important role in response to cadmium stress. This study provides a theoretical basis for further exploring the potential mechanism of DNA methylation in response to cadmium stress in plants.