%A ZHOU Yue, ZHAO Zhi-Hua, ZHANG Hong-Ning, KONG You-Bin %T Cloning and functional analysis of the promoter of purple acid phosphatase gene GmPAP14 in soybean %0 Journal Article %D 2022 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2022.14016 %P 590-596 %V 48 %N 3 %U {https://zwxb.chinacrops.org/CN/abstract/article_7290.shtml} %8 2022-03-12 %X

The expression of GmPAP14 was induced under low phosphorus condition, and its overexpression could significantly improve the utilization efficiency of organic phosphorus in Arabidopsis. In order to further explore its regulatory mechanism, the promoter sequence of GmPAP14 was cloned according to the sequence of soybean reference genome. The regulatory elements of GmPAP14 promoter were predicted by the database PLACE and PlantCARE, which showed that it contained enhancer regulatory elements, tissue-specific expression elements, root-specific expression elements, and P1BS elements (transcription factor PHR1 binding sites). PGmPAP14-2568-GUS, PGmPAP14-2238-GUS, and PGmPAP14-1635-GUS were constructed and transferred into Arabidopsis thaliana via Floral dip method. The expressional activities of three fragments of GmPAP14 promoter were analyzed through GUS staining and activity measurement. The results revealed that GmPAP14 promoter was mainly expressed in root tip under Pi condition, and GUS staining was extended to the elongation area, mature area, and root hair after low phosphorus treatment. Additionally, Arabidopsis plants with PGmPAP14-2238-GUS had the highest activity among them. These results lay an important foundation for the further study of gene regulation.