%A SHI Yu-Qin, SUN Meng-Dan, CHEN Fan, CHENG Hong-Tao, HU Xue-Zhi, FU Li, HU Qiong, MEI De-Sheng, LI Chao %T Genome editing of BnMLO6 gene by CRISPR/Cas9 for the improvement of disease resistance in Brassica napus L %0 Journal Article %D 2022 %J Acta Agronomica Sinica %R 10.3724/SP.J.1006.2022.14077 %P 801-811 %V 48 %N 4 %U {https://zwxb.chinacrops.org/CN/abstract/article_7364.shtml} %8 2022-04-12 %X

Gene editing technology can modify the target gene efficiently and accurately, which opens up a new way for crop genetic improvement. Mildew resistance locus O (MLO) gene is a key negative regulator of plant defense against powdery mildew. Mutation of MLO gene can enhance plant resistance to powdery mildew, but whether it has the same function is not reported in oilseed rape. In this study, the relative expression analysis suggested that BnMLO6 gene was induced by Sclerotinia sclerotiorum. To explore the potential role of BnMLO6 gene in pathogen resistance, six homologous copies of BnMLO6 gene mutated synchronously by CRISPR/Cas9 gene editing technology and mlo6-212 mutant line was generated for further analysis. Genetic analysis revealed that CRISPR/Cas9 induced mutagenesis of BnMLO6 gene could be stably inherited. In addition, mlo6-212 mutant line indicated obvious resistance to powdery mildew in both field and greenhouse condition. The lesion area of mlo6-212 mutant was reduced by 19.5% after 24 hours inoculation with S. sclerotiorum. Meanwhile, mutation of BnMLO6 gene could stimulate the spontaneous accumulation of callose in leaves and activate ethylene and jasmonic acid transduction pathway. Thus, BnMLO6 gene was probably involved in multiple pathogen resistance pathways to negatively regulate resistance to powdery mildew and S. sclerotiorum in oilseed rape. The results not only provide theoretical basis for the study of BnMLO6 involved resistance regulation of multiple pathogens, but also provide resistant resources and technical support for genetic improvement of disease resistance in oilseed rape.