Characterization of Fusarium Head Blight Resistance Gene Fhb1 and Its Putative Ancestor in Chinese Wheat Germplasm
Zhan-Wang ZHU, Deng-An XU, Shun-He CHENG, Chun-Bao GAO, Xian-Chun XIA, Yuan-Feng HAO, Zhong-Hu HE
Acta Agronomica Sinica
2018, 44 ( 04):
473-482.
DOI: 10.3724/SP.J.1006.2018.00473
Enhancing resistance to Fusarium head blight (FHB) has become one of important breeding objectives in the major wheat-growing regions in China. A prominent locus Fhb1 conferring stable FHB resistance with the largest effect is the major source of resistance in wheat breeding. Understanding the distribution and putative donor of Fhb1 in Chinese wheat cultivars will facilitate the application of this gene and thus benefit FHB resistance breeding in China. Haplotype analysis of PFT (pore-forming toxin-like), HC (HCBT-like defense response protein) and His (histidine-rich calcium-binding protein) genes in the Fhb1 region of 229 wheat cultivars and advanced lines indicated that PFT-I/His-I was a resistant haplotype. Both pedigree and marker (or sequence) information revealed that Fhb1 in Chinese wheat cultivars was mainly derived from Sumai 3 and Ningmai 9, in which Ningmai 9 was the major donor. The Fhb1 diagnostic markers PFT-CAPS and His-InDel developed in this study can be used effectively in marker-assisted selection for improving FHB resistance.
品种(系)
Cultivar (line) | 来源
Origin | PFT检测方法†
PFT detection method† | His等位基因
His allele | HC等位基因
HC allele | 病情指数
FHB index | | 苏麦3号 Sumai 3 | 江苏 Jiangsu | 1, 2 | His-I | HC-I | 7.97±6.86 | | 宁7840 Ning 7840 | 江苏 Jiangsu | 1, 2 | His-I | HC-I | 20.12±17.35 | | 镇麦5号 Zhenmai 5 | 江苏 Jiangsu | 1, 2 | His-I | HC-I | 30.23±8.78 | | 宁麦9号 Ningmai 9 | 江苏 Jiangsu | 1, 2 | His-I | HC-I | 27.29±21.45 | | 宁麦13 Ningmai 13 | 江苏 Jiangsu | 1, 2 | His-I | HC-I | 46.87±21.09 | | 宁麦16 Ningmai 16 | 江苏 Jiangsu | 1, 2 | His-I | HC-I | 46.62±17.80 | | 西农9871 Xinong 9871 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 94.33±4.97 | | 小偃22 Xiaoyan 22 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 82.30±15.66 | | 西农979 Xinong 979 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 76.46±26.53 | | 荔垦2号 Liken 2 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 51.29±7.38 | | 西农88 Xinong 88 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 77.05±19.41 | | 陕麦150 Shaanmai 150 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 38.20±10.61 | | 陕159 Shaan 159 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 59.93±6.15 | | 陕253 Shaan 253 | 陕西 Shaanxi | 1 | His-II | HC-I | 65.93±6.29 | | 陕715 Shaan 715 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 60.93±21.00 | | 小偃6号 Xiaoyan 6 | 陕西 Shaanxi | 1, 2 | His-II | HC-I | 48.42±19.93 | | 小偃107 Xiaoyan 107 | 陕西 Shaanxi | 1 | His-II | HC-I | 51.48±35.45 | | 鄂07901 E07901 | 湖北 Hubei | 1 | His-II | HC-I | 47.02±35.36 | | 烟农22 Yannong 22 | 山东 Shandong | 1, 2 | His-II | HC-I | 69.33±18.41 | | 济宁16 Jining 16 | 山东 Shandong | 1, 2 | His-II | HC-I | 78.08±19.30 | | 济麦19 Jimai 19 | 山东 Shandong | 1 | His-II | HC-I | 66.55±26.55 | | 长6359 Chang 6359 | 山西 Shanxi | 1, 2 | His-II | HC-I | 60.61±33.45 | | 郑麦9023 Zhengmai 9023 | 河南 Henan | 1, 2 | His-II | HC-I | 41.14±10.44 | | 烟2415 Yan 2415 | 山东 Shandong | 1 | His-III | HC-II | 42.44±26.00 |
Table 3
Alleles of His and HC genes for the cultivars with PFT-I and their FHB indexes
Extracts from the Article
表3 PFT-I类型品种(系)所含His及HC的等位基因及其赤霉病病情指数
† 1和2分别表示PFT-CAPS标记和测序检测。† 1 and 2 refer to detection with PFT-CAPS marker and sequencing, respectively.
附表3 我国含Fhb1小麦品种(系)系谱
Other Images/Table from this Article
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Supplementary table 1
Origins of 229 wheat cultivars (lines) and their His and PFT genotypes
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Supplementary table 2
Detection of Fhb1 in 73 Yangmai and Ningmai cultivars (lines)
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Table 1
Specific primers used to amplify the open reading frames (ORF) of PFT, His, and HC
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Table 2
Analysis of variance (ANOVA) of Fusarium head blight index in 229 wheat cultivars
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Fig. 1
Structure, primer binding sites and three alleles of PFT gene
The first nucleotide of the initiation codon reads as +1
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Fig. 2
Amplification profiles of marker PFT-CAPS in partial Chinese cultivars
M: DL5000 DNA marker; 1-4: PFT-I cultivars, namely Sumai 3, Ningmai 9, Xinong 9871, and Xiaoyan 22; 5-8: PFT-II cultivars, namely Zhengmai 366, Lumai 21, Hengguan 35, and Ningchun 4. PCR products were digested with Dra I restriction endonuclease.
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Fig. 3
Structure, primer binding sites, and alleles of HC and His genes for the cultivars with PFT-I
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Fig. 4
Amplification profiles of marker His-InDel in partial Chinese cultivars
M: DL5000 DNA marker; 1-4: His-I cultivars, namely Sumai 3, Ning 7840, Ningmai 9, and Ningmai 13; 5-11: non His-I cultivars, namely Xinong 9871, Xiaoyan 22, Zhengmai 9023, Yan 2415, Yangmai 158, Jimai 22, and Aikang 58.
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Fig. 5
Average FHB indexes of cultivars or lines with different PFT/His haplotypes
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Fig. 6
Gene flow of Fhb1 in 73 cultivars (lines) of Yangmai and Ningmai through founder parent Ningmai 9
Cultivars or lines in solid boxes were characterized carrying Fhb1 using PFT-CAPS and His-InDel markers, while those in dotted boxes were uncharacterized.
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Supplementary table 3
Supplementary table 3 Pedigrees of Chinese wheat cultivars (lines) carrying Fhb1
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