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Improvement of Resistance of Wheat Cultivars to Fusarium Head Blight in the Yellow-Huai Rivers Valley Winter Wheat Zone with Functional Marker Selection of Fhb1 Gene
Hong-Jun ZHANG, Zhen-Qi SU, Gui-Hua BAI, Xu ZHANG, Hong-Xiang MA, Teng LI, Yun DENG, Chun-Yan MAI, Li-Qiang YU, Hong-Wei LIU, Li YANG, Hong-Jie LI, Yang ZHOU
Acta Agronomica Sinica    2018, 44 (04): 505-511.   DOI: 10.3724/SP.J.1006.2018.00505
Abstract   (775 HTML16 PDF(pc) (3899KB)(689)  

Fusarium head blight (FHB) becomes a major disease in the wheat production of the Yellow-Huai Rivers Valley Winter Wheat Zone of China. Improving FHB resistance is one of the major breeding targets in this region. The donors of Fhb1 gene, including Ningmai 9, Shengxuan 6, Jianyang 798, Jianyang 84, Sumai 3, and Ningmai 13, were moderately or highly resistant to FHB. Six backcrossing populations were developed by crossing these donors with a dwarfing and male-sterile wheat near isogenic line Zhoumai 16, and backcrossing with Zhoumai 16. The progenies were subjected to phenotypic evaluation of FHB by both the floret-inoculation method and natural infection in a field disease nursery. Fhb1 was detected by a functional Kompetitive Allele Specific PCR (KASP) marker. The backcrossing progenies with Fhb1 gene were moderately susceptible to FHB. Compared with the backcrossing progenies without Fhb1 gene, mean number of diseased spikelets and disease index reduced by 4.2 (P < 0.01) and 4.0 in the progenies with Fhb1 gene, respectively. The mean number of diseased spikelets and disease index for the backcrossing progenies with Fhb1 gene were 8.1 (P < 0.01) and 28.4 (P < 0.01) lower than those of the recurrent parent Zhoumai 16. There were significant differences in resistance among the backcrossing progenies from different donors. The progenies from Shengxuan 6 had better performance in number of diseased spikelets and disease index compared with those from any other donors. The results from this study indicate that Fhb1 gene can efficiently improve the FHB resistance of wheat cultivars grown in the Yellow-Huai Rivers Valley Winter Wheat Zone.


Fig. 1 Performance of FHB resistance in the backcrossing progenies with or without Fhb1 gene from different donors, recurrent parent and controls using the floret-inoculation method
The number of diseased spikelets of backcrossing progenies with Fhb1 gene from donors Ningmai 9 (NM 9, A), Shengxuan 6 (SX 6, B), Jianyang 798 (JY 798, C), Jianyang 84 (JY 84, D), Sumai 3 (SM 3, E), and Ningmai 13 (NM 13, F) were obviously lower than the backcrossing progenies without Fhb1 gene. The recurrent parent Zhoumai 16 (ZM 16) and the highly susceptible control Annong 8455 (AN 8455) had more number of diseased spikelets than Huaimai 20 (HM 20), the moderately susceptible control (G) and Yangmai 158 (YM 158), the moderately resistant control (H). +: Fhb1 genotype; –: non-Fhb1 genotype; RP: recurrent parent; MS: moderately susceptible; HS: highly susceptible; MR: moderately resistant.
Extracts from the Article
图1   单花滴注接种鉴定携带和不携带供体Fhb1基因的回交后代、轮回亲本及对照赤霉病抗性表现
携带宁麦9号(A)、生选6号(B)、建阳798 (C)、建阳84 (D)、苏麦3号(E)和宁麦13 (F) Fhb1基因回交后代的病小穗数明显少于不携带供体Fhb1基因的回交后代。轮回亲本周麦16较中感对照淮麦20 (G)、高感对照安农8455较中抗对照扬麦158 (H)具有更多的病小穗数。+: 携带Fhb1基因; –: 不携带Fhb1基因; RP: 轮回亲本; MS: 中感; HS: 高感; MR: 中抗。
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