抗小麦黄矮病相关蛋白激酶TiDPK1与BYDV外壳蛋白的互作
汪信东, 陈亮, 张增艳*
中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 / 农业部麦类生物学与遗传育种重点实验室, 北京 100081
抗小麦黄矮病相关蛋白激酶TiDPK1与BYDV外壳蛋白的互作
WANG Xin-Dong, CHEN Liang, ZHANG Zeng-Yan*
图3 酵母双杂交系统检验TiDPK1与BYGA-GAV CP-PAV CP互作 1: 转化实验组质粒pGADT7-TiDPK1+pGBKT7-GAV-CP, pGADT7-TiDPK1+pGBKT7-PAV-CP的酵母在四缺营养选择培养基上生长良好, 在X-α-gal定性实验变蓝; 2和3: 转化对照组质粒的酵母pGADT7-TiDPK1+pGBKT7、pGADT7+pGBKT7-GAV-CP和pGADT7+pGBKT7-PAV-CP不能在四缺培养基上生长, 同时X-α-gal定性实验无颜色变化。
Fig. 3 BYDV-GAV CP-PAV CP interacts with TiDPK1 in the YTH system 1: Yeast colonies containing pGBKT7-CPpGADT7-TiDPK1 grew well on SD-Ade-His-Leu-Trp medium and turned blue in X-α-gal assays; 2 and 3: Yeast colonies containing controls plasmids unable to grow on SD-Ade-His-Leu-Trp medium and turned blue in X-α-gal assays.
Fig. 3 BYDV-GAV CP-PAV CP interacts with TiDPK1 in the YTH system 1: Yeast colonies containing pGBKT7-CPpGADT7-TiDPK1 grew well on SD-Ade-His-Leu-Trp medium and turned blue in X-α-gal assays; 2 and 3: Yeast colonies containing controls plasmids unable to grow on SD-Ade-His-Leu-Trp medium and turned blue in X-α-gal assays.