水稻Ef7 基因的一个新等位基因Ef7-l 的遗传效应及表达分析
赵冬生, 张昌泉, 顾铭洪, 刘巧泉*
扬州大学农学院 / 江苏省作物遗传生理重点实验室 / 教育部植物功能基因组学重点实验室, 江苏扬州 225009
ZHAO Dong-Sheng, ZHANG Chang-Quan, GU Ming-Hong, LIU Qiao-Quan*
图3 Ef7 基因在日本晴和CL63中的表达模式 a: Ef7 基因在日本晴植株不同器官中的表达; R: 根; S: 茎秆; LB: 叶片; LS: 叶鞘; P: 抽穗前的幼穗; 5P: 开花后5 d的幼穗; 10P: 开花后10 d的穗; 15P: 开花后15 d的穗; 20P: 开花后20 d的穗。 b: Ef7 基因长日照条件下的表达模式; c: Ef7 基因在短日照条件下的表达模式。表达水平以相对于持家基因 Actin 的表达水平衡量; ST表示一天中的取样时间; 黑色实圈表示日本晴, 白色空圈表示CL63。
Fig. 3 Expression pattern of the Ef7 gene in Nipponbare and CL63 a: Ef7 gene expression of various organs of Nipponbare; R: root, S: stem, LB: leaf blade, LS: leaf sheath, P: young panicle before heading, 5P: young panicle 5 days after flowering, 10P: panicle 10 days after flowering, 15P: panicle 15 days after flowering, 20P: panicle 20 days after flowering. b: Expression level of Ef7 between Nipponbare and CL63 under LD conditions; c: Expression level of Ef7 between Nipponbare and CL63 under SD conditions. Expression level was normalized against expression of Actin gene. ST: sampling time in one day. Solid dark circles represent Nipponbare, and open white circles represent CL63.
Fig. 3 Expression pattern of the Ef7 gene in Nipponbare and CL63 a: Ef7 gene expression of various organs of Nipponbare; R: root, S: stem, LB: leaf blade, LS: leaf sheath, P: young panicle before heading, 5P: young panicle 5 days after flowering, 10P: panicle 10 days after flowering, 15P: panicle 15 days after flowering, 20P: panicle 20 days after flowering. b: Expression level of Ef7 between Nipponbare and CL63 under LD conditions; c: Expression level of Ef7 between Nipponbare and CL63 under SD conditions. Expression level was normalized against expression of Actin gene. ST: sampling time in one day. Solid dark circles represent Nipponbare, and open white circles represent CL63.