广谱稻瘟病抗性基因<italic>Pigm</italic> 和<italic>Pi2</italic> 的抗谱差异及与<italic>Pi1</italic> 的互作效应

广谱稻瘟病抗性基因Pigm 和Pi2 的抗谱差异及与Pi1 的互作效应

于苗苗^1,^2,³, 戴正元¹, 潘存红¹, 陈夕军², 余玲¹, 张晓祥¹, 李育红¹, 肖宁¹, 龚红兵³, 盛生兰³, 潘学彪², 张洪熙¹, 李爱宏^1,^*

Resistance Spectrum Difference between Two Broad-Spectrum Blast Resistance Genes,Pigm andPi2 , and Their Interaction Effect onPi1

YU Miao-Miao^1,^2,³, DAI Zheng-Yuan¹, PAN Cun-Hong¹, CHEN Xi-Jun², YU Ling¹, ZHANG Xiao-Xiang¹, LI Yu-Hong¹, XIAO Ning¹, GONG Hong-Bing³, SHENG Sheng-Lan³, PAN Xue-Biao², ZHANG Hong-Xi¹, LI Ai-Hong^1,^*

图1 目标基因近等基因系的构建 A: 目标基因BC 4 F 2 世代的选择, 绿色箭头示目标个体M: DL2000 DNA标准分子量; P1: 受体亲本; P2: 供体亲本; L1~17: BC 4 F 2 世代不同基因型个体; B: 标记选择为阳性的株系进行苗瘟接种验证, 红色箭头示携带目标基因的抗性株系, 白色箭头示感病轮回亲本; C: 携带目标基因的株系叶片呈现过敏性坏死病班抗性表型; D: 轮回亲本叶片呈现感病表型。
Fig. 1 Construction of NILs carrying target genes A: choosing of target genes in BC 4 F 2 generation, and green arrows indicate target individuals M: DL2000 DNA marker; P1: donor parent; P2: recipient parent; L1-17: Individuals with different genotypes in BC 4 F 2 generation. B: positive lines were inoculated on seedling stage, and red arrows show the resistant lines carrying target genes, while white arrows indicate susceptible parents. C: line carrying the target gene shows resistant phenotype. D: recurrent parent shows susceptible phenotype.