拟南芥G蛋白α亚基GPA1互作蛋白铜离子结合蛋白AtBCB的鉴定及功能分析

张小红¹, 许鹏博^1,², 郭萌萌^1,², 徐兆师², 李连城², 陈明^2,^*, 马有志²

Characteristic and Function Analysis of a Copper Ion Binding Protein, AtBCB Interacting with G Protein α Subunit GPA1 inArabidopsis thaliana

ZHANG Xiao-Hong¹, XU Peng-Bo^1,², GUO Meng-Meng^1,², XU Zhao-Shi², LI Lian-Cheng², CHEN Ming^2,^*, MA You-Zhi²

图1 用泛素分离系统验证GPA1与AtBCB的相互作用 GPA1-Cub和AtBCB-NubG及阴性对照共转化酵母感受态细胞, 左图为SD-Trp-Leu选择性培养基生长结果; 右图为SD-Trp-Leu-His-Ade+1.5 mmol L -1 3-AT+40 mg L -1 X-α-gal选择性培养基生长结果。10 0 , 10 -1 , 10 -2 代表不同的稀释浓度。
Fig. 1 Identification of interaction of GPA1 and AtBCB by split-ubiquitin system GPA1-Cub and AtBCB-NubG were co-transformed into yeast competent cells. Left: yeasts grown on SD-Trp-Leu; Right: yeats grown on SD-Trp-Leu-His-Ade+1.5 mmol L -1 3-AT+40 mg L -1 X-α-gal. 10 0 , 10 -1 , 10 -2 : different concentration gradients.