拟南芥G蛋白α亚基GPA1互作蛋白铜离子结合蛋白AtBCB的鉴定及功能分析

张小红¹, 许鹏博^1,², 郭萌萌^1,², 徐兆师², 李连城², 陈明^2,^*, 马有志²

Characteristic and Function Analysis of a Copper Ion Binding Protein, AtBCB Interacting with G Protein α Subunit GPA1 inArabidopsis thaliana

ZHANG Xiao-Hong¹, XU Peng-Bo^1,², GUO Meng-Meng^1,², XU Zhao-Shi², LI Lian-Cheng², CHEN Ming^2,^*, MA You-Zhi²

图3 突变体纯合体鉴定 A: bcb 纯合体鉴定; B: gpa1-4 纯合体鉴定; M为DL2000; LP和RP为扩增目的基因的正反向引物; LB为T-DNA插入的左边界引物; WT为野生型, 其余为突变体。
Fig. 3 Identification of homozygous mutants A: verification of gpa1-4 homozygous mutant; B: verification of bcb homozygous mutant; M: DL2000; LP and RP: forward and reverse primer of target genes; LB: left border primer of the T-DNA insertion; WT: wild type and others are mutants.