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转TiERF1-RC7 双价基因小麦的鉴定及其全蚀病抗性
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刘菲1, 杨丽华1,2, 王爱云3, 刘欣2, 马小飞1, 杜丽璞2, 李盼松1, 张增艳2,*, 马翎健1,* |
Molecular Detection and Take-all Response Assays ofTiERF1-RC7 Transgenic Wheat
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LIU Fei 1, YANG Li-Hua 1,2, WANG Ai-Yun 3, LIU Xin 2, MA Xiao-Fei 1, DU Li-Pu 2, LI Pan-Song 1, ZHANG Zeng-Yan 2,*, MA Ling-Jian 1,*
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图3 小麦全蚀病菌菌丝侵染转基因小麦及受体扬麦18根表的扫描电镜观察 A和B: 接种48 h转基因小麦10ZGY-92和扬麦18根表面有少量菌丝48 h; C和D: 接种60 h菌丝在10ZGY-92和扬麦18的根毛上缠绕, 与受体小麦扬麦18 D相比, 抗病转基因小麦10ZGY-92细胞表面的 Ggt 菌丝数量少且菌丝细C; E: 接种120 h菌丝未进入转基因小麦10ZGY-92的中柱; F: 接种120 h后菌丝穿过扬麦18的中柱。 Fig. 3 Scanning electron microscope observation of invasion on the root surfaces of the transgenic and Yangmai 18 plants by take-all pathogen A and B: a small amount of hyphae was on the root surfaces of transgenic wheat 10ZGY-92 and Yangmai 18 at inoculation for 48 h; C and D: mycelium winded root hairs in transgenic wheat 10ZGY-92 and Yangmai 18 at inoculation for 60 h; Ggt was less and thinner in resistant transgenic wheat 10ZGY-92 than in Yangmai 18; E: no mycelium invaded columns in transgenic wheat 10ZGY-92 at inoculation for120 h; F: mycelium invaded column layer in Yangmai 18 at inoculation for 120 h. |
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