大豆谷氨酰胺合成酶基因的分类及根瘤特异表达<italic>GmGS1β2</italic>基因功能的初步分析

大豆谷氨酰胺合成酶基因的分类及根瘤特异表达GmGS1β2基因功能的初步分析

王晓波¹, 滕婉², 何雪², 童依平^2,^*

Classification of Glutamine Synthetase Gene and Preliminary Functional Analysis of the Nodule-Predominantly Expressed GeneGmGS1β2 in Soybean

WANG Xiao-Bo¹, TENG Wan², HE Xue², TONG Yi-Ping^2,^*

图4 GmGS1β2重组蛋白纯化与谷氨酰胺合成酶活性分析 A: pGEX4T-1-GmGS1β2纯化后表达产物SDS-PAGE分析; M: 蛋白质分子质量标准; 1: 纯化后pGEX4T-1空载体诱导表达产物GST; 2: 纯化后pGEX4T-1-GmGS1β2重组质粒诱导表达产物; 箭头示目标条带; B: GmGS1β2重组蛋白可以催化底物生成红褐色产物; 1: GST标签蛋白GS催化反应; 2: GST:GmGS1β2重组蛋白GS催化反应; C: GmGS1β2重组蛋白的GS活性。
Fig. 4 Purification and Glutamine synthetase activity analysis of GmGS1β2 recombinant protein A: SDS-PAGE analysis of the purified expressed product of pGEX4T-1-GmGS1β2; M: protein molecular weight standard; 1: purified proteins of strains harboring pGEX4T-1GST; 2: purified proteins of engineering bacteria strains pGEX4T-1-GmGS1β2; Arrow indicates the target protein band; B: catalyzing reaction of GmGS1β2 recombinant protein to its substrate by producing red-brown productcatalytic reaction of 1 for GST peptide tag and 2 for GST:GmGS1β2 recombinant protein; C: GS activity analysis for GST peptide tag and GST:GmGS1β2 recombinant protein.