甘蔗ABA信号转导关键酶<italic>SoSnRK2.1</italic>基因的克隆与表达分析

甘蔗ABA信号转导关键酶SoSnRK2.1基因的克隆与表达分析

谭秦亮¹, 李长宁^1,², 杨丽涛^1,^2,^*, 李杨瑞^1,^2,^*

Cloning and Expression Analysis of Abscisic Acid Signal Transduction Key Enzyme GeneSoSnRK2.1 from Sugarcane

TAN Qin-Liang¹, LI Chang-Ning^1,², YANG Li-Tao^1,^2,^*, LI Yang-Rui^1,^2,^*

图7 SoSnRK2.1 基因的原核表达 M: 蛋白marker; 1: pET-30a+未诱导; 2: pET-30a+诱导4 h; 3: pET-SoSnRK2.1未诱导; 4~7: pET-SoSnRK2.1 1.0 mmol L -1 IPTG诱导2 h、4 h、6 h、8 h。
Fig. 7 Expression of recombinant SoSnRK2.1 protein M: protein marker; 1: pET-30a+ without induction; 2: pET-30a+ with induction 4 hours; 3: pET-SoSnRK2.1 without induction; 4-7: pET-SoSnRK2.1 1.0 mmol L -1 IPTG with induction for 2, 4, 6, and 8 h, respectively.