拟南芥H<sup>+</sup>-焦磷酸化酶AVP1互作小GTP结合蛋白AtRAB的特性鉴定与功能分析

拟南芥H⁺-焦磷酸化酶AVP1互作小GTP结合蛋白AtRAB的特性鉴定与功能分析

刘荣榜^1,², 陈明^2,^*, 郭萌萌², 司青林², 高世庆³, 徐兆师², 马有志², 尹钧^1,^*

Characterization and Functional Analysis of a Small GTP-binding Protein AtRAB Interacting with H⁺-pyrophosphatase AVP1 inArabidopsis thaliana

LIU Rong-Bang^1,², CHEN Ming^2,^*, GUO Meng-Meng², SI Qing-Lin², GAO Shi-Qing³, XU Zhao-Shi², LI Lian-Cheng², MA You-Zhi², YIN Jun^1,^*

图1 酵母双杂交验证AtRAB与AVP1的相互作用 A: 将pBT 3 STE-AVP1和pPR 3 N-AtRAB及其阴性对照共转化酵母感受态细胞, 左图为双缺培养基SD-Trp-Leu的生长结果; 右图为四缺筛选培养基SD-Trp-Leu-His-Ade+40 mg L -1 X-#cod#x003b1;-gal选择性培养基生长结果, 10、10 -1 和10 -2 为不同的稀释梯度; B: 试剂盒自带质粒空载体阴阳性对照。
Fig. 1 Identification of interaction between AtRAB and AVP1 by yeast two-hybrid system A: pBT 3 STE-AVP1 and pPR 3 N-AtRAB were co-transformed into yeast competent cells. Left: yeasts grown on medium SD-Trp- Leu; Right: yeats grown on medium SD-Trp-Leu-His-Ade+40 mg L -1 X-#cod#x003b1;-gal. 10, 10 -1 , 10 -2 : different concentration gradients; B: negative and positive control.