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花生属人工异源多倍体进化早期基因表达变化的cDNA-SCoT分析
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贺梁琼1,2,3, 熊发前1, 唐秀梅1, 蒋菁1, 韩柱强1, 钟瑞春1, 高忠奎4, 李忠1, 何新华2,*, 唐荣华1,* |
Expression Variation of Genes in Early Period ofArachisArtificial Allopolypoidy Evolution Using cDNA-SCoT Technique
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HE Liang-Qiong 1,2,3, XIONG Fa-Qian 1, TANG Xiu-Mei 1, JIANG Jing 1, HAN Zhu-Qiang 1, ZHONG Rui-Chun 1, GAO Zhong-Kui 4, Li Zhong 1, HE Xin-Hua 2,*, TANG Rong-Hua 1,*
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图3 引物SCoT94在花生属种间杂种F 1 、异源多倍体早期世代S 0 ~S 3 及亲本的cDNA-SCoT扩增 a: 父本转录物完全沉默; c: 母本转录物在后代部分材料中沉默; d, e: 亲本转录物在后代部分材料中沉默; b, f: 新转录物激活。 M: DL2000 marker; Fe: 母本; Ma: 父本。 Fig. 3 cDNA-SCoT amplification result of F 1 hybrid, synthesized allopolyploid S 0 -S 3 and their progenitors in genus Arachis interspecific hybridization by primer SCoT94 a: transcript silencing completely from male parentage; c: transcript silencing from female in some materials; e, d: transcript silencing from male in some materials; b, f: expression of novel transcript. M: DL2000 marker; Fe: female parent; Ma: male parent. |
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