|
花生属人工异源多倍体进化早期基因表达变化的cDNA-SCoT分析
|
贺梁琼1,2,3, 熊发前1, 唐秀梅1, 蒋菁1, 韩柱强1, 钟瑞春1, 高忠奎4, 李忠1, 何新华2,*, 唐荣华1,* |
Expression Variation of Genes in Early Period ofArachisArtificial Allopolypoidy Evolution Using cDNA-SCoT Technique
|
HE Liang-Qiong 1,2,3, XIONG Fa-Qian 1, TANG Xiu-Mei 1, JIANG Jing 1, HAN Zhu-Qiang 1, ZHONG Rui-Chun 1, GAO Zhong-Kui 4, Li Zhong 1, HE Xin-Hua 2,*, TANG Rong-Hua 1,*
|
|
图4 引物SCoT71在花生属种间杂种F 1 、异源多倍体早期世代S 0 ~S 3 及亲本的cDNA-SCoT扩增 f: 母亲转录物完全沉默: b, e: 父本转录物在后代部分材料中沉默; c: 双亲转录都在后代部分材料中沉默; a, d: 新转录物激活; M: DL2000 marker; Fe: 母本; Ma: 父本。 Fig. 4 cDNA-SCoT amplification result of F 1 hybrid, synthesized allopolyploid S 0 -S 3 and their progenitors in genus Arachis interspecific hybridization by primer SCoT71 f: transcript silencing absolutely from female; b, e: transcript silencing from male in some materials; c: transcript silencing from parents in some materials; d: expression of novel transcript; M: DL2000 marker; Fe: female parent; Ma: male parent. |
|
|
|
|
|