籽粒苋AhNAD-ME的序列特征与表达
白云凤1,3,#cod#x0002A;, 聂江婷2, 张忠梁1, 李平1, 张维锋1, 闫建俊1, 冯瑞云1, 张耀1

Sequence Characteristics and Expression of NAD-malic enzyme inAmaranthus hypochondriacusL.
BAI Yun-Feng1,3,#cod#x0002A;, NIE Jiang-Ting2, ZHANG Zhong-Liang1, LI Ping1, ZHANG Wei-Feng1, YAN Jian-Jun1, FENG Rui-Yun1, ZHANG Yao1
图4 图 AhNAD-ME 原核表达SDS-PAGE电泳 M: 蛋白质分子量标准; 1: 不含重组质粒的菌株Transette DE3 在30℃诱导2 h后的表达产物; 2~9: 含有重组质粒的菌株Transette DE3的表达产物。其中2、3、4和5分别为30℃诱导0、2、4和6 h后的表达产物; 6和7为30℃诱导4 h后的沉淀和上清; 8和9为16℃诱导4 h后的沉淀和上清液。
Fig. 4 SDS-PAGE analysis of the AhNAD-ME expression M: premixed protein marker; 1 refers to Transette DE3 without recombined plasmid induced by IPTG under 30℃ for 2 h; 2, 3, 4, and 5 refer to Transette DE3 with recombined plasmid induced by IPTG for 0, 2, 4, and 6 hours, respectively; 6 and 7 refer to precipitate and supernatant liquor of Transette DE3 with recombined plasmid induced by IPTG under 30℃ for 4 hours, respectively; 8 and 9 refer to precipitate and supernatant liquor of Transette DE3 with recombined plasmid induced by IPTG under 16℃ for 4 hours, respectively.