棉花叶肉原生质体分离及目标基因瞬时表达体系的建立

李妮娜, 丁林云, 张志远, 郭旺珍^*

Isolation of Mesophyll Protoplast and Establishment of Gene Transient Expression System in Cotton

LI Ni-Na, DING Lin-Yun, ZHANG Zhi-Yuan, GUO Wang-Zhen^*

图6 棉花叶片原生质体的分离与纯化 a: 自然生长12 d的棉花植株; b: 棉花子叶; c: 酶解前物理处理后的棉花叶片; d: 酶解结束后酶解混合液; e: 重悬浮原生质体; f: 沉淀后原生质体箭头所示; g: 物镜下的镜检原生质体#cod#x000d7;10。
Fig. 6 Isolation and purification of cotton mesophyll protoplast a: Healthy 12-day-old cotton plant; b: Cotton cotyledon for protoplast isolation; c. Cotton leaves after physical cutting; d. Mixed solution after enzymatic digestion; e: Resuspension of cotton protoplast; f: Precipitation of cotton protoplast marked with arrow; g: The purified mesophyll protoplast #cod#x000d7;10.