棉花叶肉原生质体分离及目标基因瞬时表达体系的建立
李妮娜, 丁林云, 张志远, 郭旺珍*

Isolation of Mesophyll Protoplast and Establishment of Gene Transient Expression System in Cotton
LI Ni-Na, DING Lin-Yun, ZHANG Zhi-Yuan, GUO Wang-Zhen*
图7 pJIT166-GFP载体转化棉花原生质 pJIT166-GFP: GFP荧光; Chlorophyll: 叶绿体自发荧光; Merge: 自发荧光与GFP荧光叠加; Bright field: 明场下细胞状态。
Fig. 7 Cotton protoplast transformed by using pJIT166-GFP vector pJIT166-GFP: transformed cells with GFP fluorescence; Chlorophyll: autofluorescence of chloroplast; Merge: overlapping between autofluorescence and GFP fluorescence; Bright field: cotton protoplast cells in bright field.