棉花叶肉原生质体分离及目标基因瞬时表达体系的建立
李妮娜, 丁林云, 张志远, 郭旺珍*

Isolation of Mesophyll Protoplast and Establishment of Gene Transient Expression System in Cotton
LI Ni-Na, DING Lin-Yun, ZHANG Zhi-Yuan, GUO Wang-Zhen*
图8 35S:GFP与35S:ZFP2:GFP质粒DNA在棉花原生质体细胞瞬时表达 GFP: GFP荧光; Chlorophyll: 叶绿体自发荧光; Merge: 自发荧光与GFP荧光叠加; Bright field: 明场下细胞状态; 35S:GFP:GFP空载体; 35S:ZFP2: GFP: 带有ZFP2目的基因的质粒。
Fig. 8 Transient expression of plasmid DNAs of 35S:GFP and 35S:ZFP2:GFP in cotton protoplast GFP: transformed cells with GFP fluorescence; Chlorophyll: autofluorescence of chloroplast; Merge: overlapping between autofluorescence and GFP fluorescence; Bright field: cotton protoplast cells in bright field; 35S:GFP: GFP empty vector; 35S:ZFP2: GFP: plasmid with ZFP2 target gene.