玉米胚乳细胞原生质体的分离与流式纯化

郭艳萍^*, 任成杰^*, 李志伟, 王文斌, 张仁和, 路海东, 刘建兴华, 薛吉全^*, 郭东伟^*

Isolation and Flow Purification of Endosperm Protoplast from Developing Seed of Maize

GUO Yan-Ping^**, REN Cheng-Jie^**, LI Zhi-Wei, WANG Wen-Bin, ZHANG Ren-He, LU Hai-Dong, LIU Jian-Chao, ZHANG Xing-Hua, XUE Ji-Quan^*, GUO Dong-Wei^*

图1 不同酶浓度及组合对原生质体产量与活力的影响 1: 0.3%离析酶+0.5%纤维素酶+0.5%半纤维素酶; 2: 0.3%离析酶+1.0%纤维素酶+0.5%半纤维素酶; 3: 0.3%离析酶+0.5%纤维素酶+1.0%半纤维素酶; 4: 0.3%离析酶+1.0%纤维素酶+1.0%半纤维素酶; 5: 0.5%离析酶+0.5%纤维素酶+0.5%半纤维素酶; 6: 0.5%离析酶+1.0%纤维素酶+0.5%半纤维素酶; 7: 0.5%离析酶+0.5%纤维素酶+1.0%半纤维素酶; 8: 0.5%离析酶+1.0%纤维素酶+1.0%半纤维素酶。
Fig. 1 Effect of different enzyme concentrations and composition on the yield and vigor of protoplasts 1: 0.3% macerozyme+0.5% cellulase+0.5% hemicellulase; 2: 0.3% macerozyme+1.0% cellulase+0.5% hemicellulase; 3: 0.3% macerozyme+0.5% cellulase+1.0% hemicellulase; 4: 0.3% macerozyme+1.0% cellulase+1.0% hemicellulase; 5: 0.5% macerozyme+0.5% cellulase+0.5% hemicellulase; 6: 0.5% macerozyme+1.0% cellulase+0.5% hemicellulase; 7: 0.5% macerozyme+0.5% cellulase+1.0% hemicellulase; 8: 0.5% macerozyme+1.0% cellulase+1.0% hemicellulase.